Institute of Composite and Biomedical Materials, National Research Council, P.le Tecchio 80, 80125 Naples, Italy.
Biomacromolecules. 2010 Sep 13;11(9):2238-46. doi: 10.1021/bm100221h.
The design of functionalized polymers that can elicit specific biological responses and the development of methods to fabricate new devices that incorporate biological cues are of great interest to the biomedical community. The realization of nanostructured matrices that exhibit biological properties and that comprise fibers with diameters of similar scale to those of the natural extracellular matrix (ECM) would enable the provision of tailored materials for tissue engineering. Accordingly, the goal of this work is to create a biologically active functionalized electrospun matrix capable of guiding neurite growth for the regeneration of nerve tissue. In this study, nanoscale electrospun membranes made of poly ε-caprolactone enhanced with gelatin from calf skin were investigated to validate their biological response under in vitro culture of PC-12 nerve cells. Preliminary observations from SEM studies supported by image analysis highlighted the nanoscale texture of the scaffold with fiber diameters equal to 0.548 ± 0.140 μm. In addition, contact angle measurements confirmed the hydrophilic behavior of the membranes, ascribable to the gelatin content. We demonstrate that the balance of morphological and biochemical properties improves all the fundamental biological events of nerve regeneration, enhancing cell adhesion, proliferation, and differentiation in comparison with PCL nanofibrous scaffolds, as well as supporting the neurite outgrowth.
设计能够引发特定生物反应的功能化聚合物，并开发制造新设备的方法，这些设备将整合生物线索，这对生物医学领域具有重要意义。实现具有生物特性的纳米结构基质，并包含与天然细胞外基质（ECM）相似直径的纤维，将能够为组织工程提供定制材料。因此，本工作的目标是创建一种具有生物活性的功能化电纺基质，能够引导神经突生长，从而促进神经组织的再生。在这项研究中，研究了用小牛皮明胶增强的聚己内酯纳米级电纺膜，以验证其在 PC-12 神经细胞体外培养下的生物反应。通过 SEM 研究和图像分析得到的初步观察结果表明，支架具有纳米级纹理，纤维直径等于 0.548 ± 0.140 μm。此外，接触角测量证实了膜的亲水性，这归因于明胶的含量。我们证明，形态和生化特性的平衡改善了神经再生的所有基本生物学事件，与 PCL 纳米纤维支架相比，增强了细胞黏附、增殖和分化，并支持神经突生长。
