Junqueira J C, Ribeiro M A, Rossoni R D, Barbosa J O, Querido S M R, Jorge A O C
Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, São Paulo State University/UNESP, São José dos Campos, São Paulo, Brazil.
Photomed Laser Surg. 2010 Aug;28 Suppl 1:S67-72. doi: 10.1089/pho.2009.2526.
This study investigated in vitro the photodynamic antimicrobial effects of the photosensitizer malachite green on clinical strains of Staphylococcus, Enterobacteriaceae, and Candida.
Thirty-six microbial strains isolated from the oral cavity of patients undergoing prolonged antibiotic therapy, including 12 Staphylococcus, 12 Enterobacteriaceae, and 12 Candida strains, were studied. The number of cells of each microorganism was standardized to 10(6) cells/mL. Twenty-four assays were carried out for each strain according to the following experimental conditions: gallium-aluminum-arsenide laser and photosensitizer (n = 6, L+P+), laser and physiologic solution (n = 6, L+P-), photosensitizer (n = 6, L-P+), and physiologic solution (n = 6, L-P-). Next, cultures were prepared on brain-heart infusion agar for the growth of Staphylococcus and Enterobacteriaceae, and on Sabouraud dextrose agar for the growth of Candida, and incubated for 48 h at 37 degrees C. The results are reported as the number of colony-forming units (CFU/mL) and were analyzed with analysis of variance and the Tukey test.
The Staphylococcus, enterobacterial, and Candida strains were sensitive to photodynamic therapy with malachite green (L+P+). A reduction of approximately 7 log(10) for Staphylococcus, 6 log(10) for enterobacteria, and 0.5 log(10) for the genus Candida. Significant statistical differences were observed between the L+P+ groups and the control groups (L-P-).
The Staphylococcus, Enterobacteriaceae, and Candida strains studied were sensitive to photodynamic therapy with malachite green.
本研究在体外调查了光敏剂孔雀石绿对葡萄球菌、肠杆菌科细菌和念珠菌临床菌株的光动力抗菌作用。
研究了从接受长期抗生素治疗患者口腔中分离出的36株微生物菌株，包括12株葡萄球菌、12株肠杆菌科细菌和12株念珠菌菌株。将每种微生物的细胞数量标准化为10(6)个细胞/毫升。根据以下实验条件对每个菌株进行24次测定：砷化镓铝激光和光敏剂（n = 6，L+P+）、激光和生理溶液（n = 6，L+P-）、光敏剂（n = 6，L-P+）和生理溶液（n = 6，L-P-）。接下来，在脑心浸液琼脂上制备用于葡萄球菌和肠杆菌科细菌生长的培养物，在沙氏葡萄糖琼脂上制备用于念珠菌生长的培养物，并在37℃下孵育48小时。结果以菌落形成单位（CFU/毫升）的数量报告，并采用方差分析和Tukey检验进行分析。
葡萄球菌、肠杆菌科细菌和念珠菌菌株对孔雀石绿光动力疗法（L+P+）敏感。葡萄球菌减少约7 log(10)，肠杆菌减少6 log(10)，念珠菌属减少0.5 log(10)。在L+P+组和对照组（L-P-）之间观察到显著的统计学差异。
所研究的葡萄球菌、肠杆菌科细菌和念珠菌菌株对孔雀石绿光动力疗法敏感。
