Rosa Luciano Pereira, da Silva Francine Cristina, Nader Sumaia Alves, Meira Giselle Andrade, Viana Magda Souza
Multidisciplinary Health Institute, Federal University of Bahia, Rua Rio de Contas, 58, Candeias, Vitória da Conquista, BA, 45029094, Brazil,
Lasers Med Sci. 2014 Nov;29(6):1959-65. doi: 10.1007/s10103-014-1613-5. Epub 2014 Jun 17.
The aim of this study was to evaluate the in vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm visible laser combined with malachite green (MG) dye in the inactivation of Staphylococcus aureus (ATCC 25923) biofilms formed within compact and cancellous bone specimens. Specimens of 80 compact bones and 80 cancellous bones were contaminated with a standard suspension of S. aureus and incubated for 14 days at 37 °C to allow for the formation of biofilms. The specimens were divided into the following groups (n = 10) according to the treatment conditions: PS-L - (control - no treatment), PS+L - (only MG for 5 min), PS-L + 90 (only laser irradiation for 90 s), PS-L + 180 (only laser irradiation for 180 s), PS-L + 300 (only laser irradiation for 300 s), APDT90 (APDT for 90 s), APDT180 (APDT for 180 s), and APDT300 (APDT for 300 s). The findings were statistically analyzed using an ANOVA 5%. All of the experimental groups were significantly different from the control group for both the compact and cancellous bone specimens. The compact bone specimens that received APDT treatment (for either 90, 180, or 300 s) showed reductions in the log10 CFU/ml of S. aureus by a magnitude of 4 log10. Cancellous bone specimens treated with 300 s of APDT showed the highest efficacy, and these specimens had a reduction in S. aureus CFU/ml by a factor of 3 log10. APDT treatment using these proposed parameters in combination with MG was effective at inactivating S. aureus biofilms in compact and cancellous bone specimens.
本研究的目的是评估使用660 nm可见激光联合孔雀石绿（MG）染料的抗菌光动力疗法（APDT）对致密骨和松质骨标本中形成的金黄色葡萄球菌（ATCC 25923）生物膜的体外灭活效果。80个致密骨标本和80个松质骨标本用金黄色葡萄球菌标准悬液污染，并在37℃下孵育14天以形成生物膜。根据治疗条件将标本分为以下几组（n = 10）：PS-L -（对照组 - 未治疗），PS+L -（仅MG处理5分钟），PS-L + 90（仅激光照射90秒），PS-L + 180（仅激光照射180秒），PS-L + 300（仅激光照射300秒），APDT90（APDT处理90秒），APDT180（APDT处理180秒）和APDT300（APDT处理300秒）。使用5%的方差分析对结果进行统计学分析。所有实验组与致密骨和松质骨标本的对照组均有显著差异。接受APDT治疗（90、180或300秒）的致密骨标本中金黄色葡萄球菌的log10 CFU/ml降低了4个log10数量级。用300秒APDT处理的松质骨标本显示出最高的疗效，这些标本中金黄色葡萄球菌CFU/ml降低了3个log10数量级。使用这些建议参数联合MG的APDT治疗对灭活致密骨和松质骨标本中的金黄色葡萄球菌生物膜有效。
