Global Research Laboratory, Department of Biochemistry, Division of Brain Korea, College of Medicine, Korea University, Seoul 136-701, Korea.
Nano Lett. 2010 Aug 11;10(8):2877-83. doi: 10.1021/nl100942p.
Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4(+) T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4(+) T lymphocytes. Fabricated SiNW arrays easily separated the CD4(+) T lymphocytes from the mouse whole splenocytes with over approximately 88% purity and demonstrated tight attachment to CD4(+) T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.
硅纳米线 (SiNWs) 为生物医学应用提供了有前途的无机纳米结构。在这里，我们报告了一种新型 SiNW 阵列的开发，该阵列旨在从细胞群体的异质混合物中分离原代 CD4(+) T 淋巴细胞。我们的系统利用了链霉亲和素 (STR)-功能化 SiNW 与生物素标记的 CD4(+) T 淋巴细胞之间的特异性高亲和力结合特性。通过扫描电子显微镜观察到，所制备的 SiNW 阵列可以轻松地将 CD4(+) T 淋巴细胞从小鼠整个脾细胞中分离出来，纯度超过约 88%，并与 CD4(+) T 淋巴细胞紧密结合。因此，我们的 STR-SiNW 阵列为特异性细胞分离提供了一种潜在的工具，并进一步提供了应用于其他生物医学应用领域的可能性。
