Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA.
J Infect Public Health. 2009;2(3):136-40. doi: 10.1016/j.jiph.2009.06.002. Epub 2009 Sep 16.
Pseudomonas aeruginosa is an opportunistic pathogen of immunocompromised hosts. This pathogen has a tendency to form biofilms on the surface of indwelling catheters leading to acute and chronic urinary tract infections that result in significant morbidity and mortality. In the present study, kinetics of interleukin-10 (IL-10) production in mouse renal tissue was studied employing experimental mouse model of acute pyelonephritis induced with planktonic and biofilm cells of P. aeruginosa. IL-10 production was found to be significantly lower in biofilm cell instilled mice compared to planktonic cell infected animals, which corroborated with higher bacterial load and tissue damage. The data suggests that downregulation of IL-10 production may be novel strategy employed by biofilm cells to cause tissue damage and hence bacterial persistence. The results of the present study may open up avenues of research that will ultimately provide the foundation for the development of preventative measures and therapeutic strategies to successfully treat P. aeruginosa biofilm infections based on the administration of anti-inflammatory agents.
铜绿假单胞菌是免疫功能低下宿主的机会性病原体。这种病原体在留置导管的表面形成生物膜的倾向，导致急性和慢性尿路感染，从而导致发病率和死亡率显著增加。在本研究中，采用实验性小鼠急性肾盂肾炎模型，研究了铜绿假单胞菌浮游细胞和生物膜细胞诱导的小鼠肾组织中白细胞介素 10（IL-10）产生的动力学。与浮游细胞感染动物相比，生物膜细胞灌注小鼠的 IL-10 产生明显降低，这与更高的细菌负荷和组织损伤相一致。数据表明，IL-10 产生的下调可能是生物膜细胞导致组织损伤和细菌持续存在的新策略。本研究的结果可能为研究提供途径，最终为开发基于抗炎药物的预防措施和治疗策略提供基础，以成功治疗铜绿假单胞菌生物膜感染。
