Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Bioresour Technol. 2010 Dec;101(24):9600-4. doi: 10.1016/j.biortech.2010.07.084. Epub 2010 Jul 24.
The mild acid pretreatment and the combination of biological pretreatment by a white rot fungus Echinodontium taxodii or a brown rot fungus Antrodia sp. 5898 with mild acid pretreatment were evaluated under different pretreatment conditions for enzymatic hydrolysis and ethanol production from water hyacinth. The combined pretreatment with E. taxodii (10 days) and 0.25% H(2)SO(4) was proved to be more effective than the sole acid pretreatment. The reducing sugar yield from enzymatic hydrolysis of co-treated water hyacinth increased 1.13-2.11 fold than that of acid-treated water hyacinth at the same conditions. The following study on separate hydrolysis and fermentation with Saccharomyces cerevisiae indicated that the ethanol yield from co-treated water hyacinth achieved 0.192 g/g of dry matter, which increased 1.34-fold than that from acid-treated water hyacinth (0.146 g/g of dry matter). This suggested that the combination of biological and mild acid pretreatment is a promising method to improve enzymatic hydrolysis and ethanol production from water hyacinth with low lignin content.
温和酸预处理以及白腐菌（Echinodontium taxodii）或褐腐菌（Antrodia sp. 5898）的生物预处理与温和酸预处理相结合，在不同预处理条件下用于从水葫芦中进行酶水解和生产乙醇。事实证明，与单独的酸预处理相比，用 E. taxodii（10 天）和 0.25% H（2）SO（4）进行联合预处理更为有效。在相同条件下，经共处理的水葫芦的酶水解还原糖产率比酸处理的水葫芦提高了 1.13-2.11 倍。随后用酿酒酵母（Saccharomyces cerevisiae）进行单独水解和发酵的研究表明，共处理水葫芦的乙醇产率达到 0.192 g/g 干物质，比酸处理水葫芦（0.146 g/g 干物质）提高了 1.34 倍。这表明，对于低木质素含量的水葫芦，生物和温和酸预处理的结合是一种提高酶水解和乙醇生产的有前途的方法。
