Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum 695019, India.
Bioresour Technol. 2010 Feb;101(3):925-30. doi: 10.1016/j.biortech.2009.08.019. Epub 2009 Sep 30.
Biomass feedstock having less competition with food crops are desirable for bio-ethanol production and such resources may not be localized geographically. A distributed production strategy is therefore more suitable for feedstock like water hyacinth with a decentralized availability. In this study, we have demonstrated the suitability of this feedstock for production of fermentable sugars using cellulases produced on site. Testing of acid and alkali pretreatment methods indicated that alkali pretreatment was more efficient in making the sample susceptible to enzyme hydrolysis. Cellulase and beta-glucosidase loading and the effect of surfactants were studied and optimized to improve saccharification. Redesigning of enzyme blends resulted in an improvement of saccharification from 57% to 71%. A crude trial on fermentation of the enzymatic hydrolysate using the common baker's yeast Saccharomyces cerevisiae yielded an ethanol concentration of 4.4 g/L.
对于生物乙醇生产而言，具有较低与粮食作物竞争的生物量原料是理想的，而且这些资源可能不会在地理上本地化。因此，对于水葫芦这种分散供应的资源，分布式生产策略更为适合。在这项研究中，我们使用现场生产的纤维素酶证明了这种原料生产可发酵糖的适用性。对酸法和碱法预处理方法的测试表明，碱预处理更有效地使样品易于酶解。研究和优化了纤维素酶和β-葡萄糖苷酶的加载量以及表面活性剂的作用，以提高糖化效果。对酶混合物进行重新设计，使糖化率从 57%提高到 71%。使用普通面包酵母酿酒酵母对酶解产物进行粗发酵试验，得到的乙醇浓度为 4.4g/L。
