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[外源褪黑素对高温胁迫下黄瓜幼苗活性氧代谢的影响]

[Effects of exogenous melatonin on active oxygen metabolism of cucumber seedlings under high temperature stress].

作者信息

Xu Xiang-dong, Sun Yan, Sun Bo, Zhang Jian, Guo Xiao-qin

机构信息

College of Horticulture, Northwest A & F University, Yangling 712100, Shannxi, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2010 May;21(5):1295-300.

Abstract

Taking the seedlings of cucumber cultivar 'Jinchun 4' as test material, this paper studied the effects of foliar application of exogenous melatonin (MT) on the active oxygen metabolism of the seedlings under high temperature stress. Under the stress, the exogenous MT could significantly decrease the leaf superoxide radical (O2-*) production rate, hydrogen peroxide (H2O2) content, cell membrane permeability (electrolyte leakage), and malonaldehyde (MDA) content, but increase the activities of superoxide radical (SOD), peroxidase (POD), and catalase (CAT), and the contents of ascorbic acid (AsA), glutathione (GSH), and soluble proteins, illustrating that pretreatment with MT could inhibit the reactive oxygen species (ROS) production, raise the antioxidative enzyme activities and antioxidant contents, and decrease the membrane lipid peroxidation and electrolyte leakage to protect the integrity of lipid membranes, being able to alleviate the damage of high temperature stress to the seedlings.

摘要

以黄瓜品种‘津春4号’幼苗为试验材料,研究了高温胁迫下叶面喷施外源褪黑素(MT)对幼苗活性氧代谢的影响。在胁迫条件下,外源MT可显著降低叶片超氧阴离子自由基(O2-*)产生速率、过氧化氢(H2O2)含量、细胞膜透性(电解质渗漏率)和丙二醛(MDA)含量,但提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性以及抗坏血酸(AsA)、谷胱甘肽(GSH)和可溶性蛋白含量,表明MT预处理可抑制活性氧(ROS)产生,提高抗氧化酶活性和抗氧化物质含量,降低膜脂过氧化和电解质渗漏,保护脂质膜的完整性,减轻高温胁迫对幼苗的伤害。

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