Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
J Am Chem Soc. 2010 Sep 8;132(35):12197-9. doi: 10.1021/ja105181v.
Fluorogenic reactions have recently emerged as a powerful tool for detection, diagnostics, and biosensing applications in a chemical and biological context. However, conventional fluorogenic systems reported to date rely on energy- or photoinduced electron transfer within the probes. Our communication demonstrates a conceptually new approach for generating a strong fluorescence signal through chemical bond formation mediated by a heavy-atom removal process. This method has favorable photophysical properties such as exceptional quantum yield and very low limits of fluorogenic DNA detection.
荧光反应最近在化学和生物学背景下的检测、诊断和生物传感应用中成为一种强大的工具。然而，迄今为止报道的传统荧光系统依赖于探针内的能量或光诱导电子转移。我们的通讯展示了一种通过重原子去除过程介导的化学键形成来产生强荧光信号的新概念方法。该方法具有优异的光物理性质，如非凡的量子产率和非常低的荧光 DNA 检测限。
