Podleski W K, Allman J R
Clinical Immunopharmacology, Allergy & Asthma, Denver, CO 80215.
Int J Immunopharmacol. 1991;13(2-3):125-8. doi: 10.1016/0192-0561(91)90088-o.
Rheumatoid arthritis (RA) is a disease of multiple etiologies and clinical evidence suggests that a separate variant called "allergic arthritis" induced by food antigens could exist. A missing link in the confirmation of such an observation is a relative lack of a reliable in vitro assay which can confirm the in vivo oral ingestion challenge. Therefore, white blood cells (WBC) from 33 rheumatoid arthritis patients were separated and their disintegration was measured in the presence of specific IgE RAST positive sera and gluten-gliadin antigens. This assay was called the antibody-dependent allergic autocytotoxicity (ACT) test which represents an equivalent of an oral ingestion challenge with food antigens. Control WBC expressed 10-45% disintegration as compared to 75-95% in RA. Preincubation of WBC with OM-89 (immunomodulating fractions of Escherichia coli, OM Laboratories Ltd, Geneva, Switzerland) inhibited significantly antibody-dependent ACT in a dose-related manner (P less than 0.001) in our patients.
类风湿性关节炎（RA）是一种多病因疾病，临床证据表明可能存在一种由食物抗原诱发的名为“过敏性关节炎”的独立变体。确认这一观察结果的一个缺失环节是相对缺乏一种能够证实体内口服摄入激发试验的可靠体外检测方法。因此，分离了33例类风湿性关节炎患者的白细胞，并在存在特异性IgE RAST阳性血清和谷蛋白-麦醇溶蛋白抗原的情况下测量其崩解情况。该检测方法被称为抗体依赖性过敏性自身细胞毒性（ACT）试验，它相当于用食物抗原进行的口服摄入激发试验。与类风湿性关节炎患者中75 - 95%的崩解率相比，对照白细胞的崩解率为10 - 45%。在我们的患者中，白细胞与OM - 89（瑞士日内瓦OM实验室有限公司的大肠杆菌免疫调节组分）预孵育以剂量相关的方式显著抑制了抗体依赖性ACT（P小于0.001）。
