Mroz M M, Kreiss K, Lezotte D C, Campbell P A, Newman L S
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colo. 80206.
J Allergy Clin Immunol. 1991 Jul;88(1):54-60. doi: 10.1016/0091-6749(91)90300-d.
The T cell response to beryllium, measured in bronchoalveolar lavage by the lymphocyte transformation test (LTT), is a critical diagnostic test for discriminating between chronic beryllium disease (CBD) and other granulomatous diseases. We examined the sensitivity, reproducibility, and methods of a less invasive, peripheral blood LTT in 17 patients with CBD and in 18 beryllium-exposed control subjects. Ninety-four percent of CBD cases (16/17) had abnormal blood LTT results, and all 18 beryllium-exposed control subjects had normal blood LTT results. Split samples for 10 beryllium disease cases and eight control subjects demonstrated that the blood LTT was reproducible between two separate laboratories. The LTT was equally sensitive with 10% and 20% serum in the culture medium. We conclude that an abnormal blood LTT can be used to diagnose CBD in patients with compatible lung pathology.
通过淋巴细胞转化试验（LTT）在支气管肺泡灌洗中检测的T细胞对铍的反应，是鉴别慢性铍病（CBD）和其他肉芽肿性疾病的关键诊断试验。我们在17例CBD患者和18例铍暴露对照受试者中检查了一种侵入性较小的外周血LTT的敏感性、可重复性及方法。94%的CBD病例（16/17）外周血LTT结果异常，所有18例铍暴露对照受试者外周血LTT结果均正常。对10例铍病病例和8例对照受试者的分装样本检测表明，外周血LTT在两个独立实验室之间具有可重复性。培养基中含10%和20%血清时，LTT的敏感性相同。我们得出结论，外周血LTT异常可用于诊断有相符肺部病理表现的患者的CBD。
