Nanobiomedical Center, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA.
Nano Lett. 2010 Sep 8;10(9):3620-7. doi: 10.1021/nl101939e.
Linear double-stranded DNA (dsDNA) viruses package their genome into a procapsid using an ATP-driven nanomotor. Here we report that bacteriophage phi29 DNA packaging motor exercises a one-way traffic property for dsDNA translocation from N-terminal entrance to C-terminal exit with a valve mechanism in DNA packaging, as demonstrated by voltage ramping, electrode polarity switching, and sedimentation force assessment. Without the use of gating control as found in other biological channels, the observed single direction dsDNA transportation provides a novel system with a natural valve to control dsDNA loading and gene delivery in bioreactors, liposomes, or high throughput DNA sequencing apparatus.
线性双链 DNA（dsDNA）病毒使用 ATP 驱动的纳米马达将其基因组包装到原衣壳中。在这里，我们报告噬菌体 phi29 DNA 包装马达在 DNA 包装中通过阀门机制行使单向 dsDNA 易位的性质，从 N 端入口到 C 端出口，这通过电压斜坡、电极极性切换和沉降力评估来证明。在没有像其他生物通道中发现的门控控制的情况下，观察到的单方向 dsDNA 运输提供了一个具有天然阀门的新型系统，用于控制生物反应器、脂质体或高通量 DNA 测序仪器中的 dsDNA 加载和基因传递。
