细菌病毒T3、T4、SPP1和Phi29的DNA包装马达共有的三步通道构象变化。
Three-step channel conformational changes common to DNA packaging motors of bacterial viruses T3, T4, SPP1, and Phi29.
作者信息
Wang Shaoying, Ji Zhouxiang, Yan Erfu, Haque Farzin, Guo Peixuan
机构信息
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Department of Physiology & Cell Biology, College of Medicine, and Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA; College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
出版信息
Virology. 2017 Jan;500:285-291. doi: 10.1016/j.virol.2016.04.015. Epub 2016 May 12.
The DNA packaging motor of dsDNA bacterial viruses contains a head-tail connector with a channel for the genome to enter during assembly and to exit during host infection. The DNA packaging motor of bacterial virus phi29 was recently reported to use the "One-way revolving" mechanism for DNA packaging. This raises a question of how dsDNA is ejected during infection if the channel acts as a one-way inward valve. Here we report a three step conformational change of the portal channel that is common among DNA translocation motors of bacterial viruses T3, T4, SPP1, and phi29. The channels of these motors exercise three discrete steps of gating, as revealed by electrophysiological assays. The data suggest that the three step channel conformational changes occur during DNA entry process, resulting in a structural transition in preparation for DNA movement in the reverse direction during ejection.
双链DNA细菌病毒的DNA包装马达包含一个头尾连接器，该连接器有一个通道，用于基因组在组装过程中进入，并在宿主感染期间退出。最近有报道称，细菌病毒phi29的DNA包装马达在DNA包装过程中使用“单向旋转”机制。这就提出了一个问题：如果该通道作为单向内向阀，那么在感染过程中双链DNA是如何被排出的。在这里，我们报道了门户通道的三步构象变化，这在细菌病毒T3、T4、SPP1和phi29的DNA转运马达中是常见的。电生理分析表明，这些马达的通道执行三个离散的门控步骤。数据表明，三步通道构象变化发生在DNA进入过程中，导致结构转变，为在排出过程中DNA反向移动做准备。
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