The Department of Food Science, University of Arkansas, Fayetteville, AR, USA.
J Appl Microbiol. 2010 Dec;109(6):1957-66. doi: 10.1111/j.1365-2672.2010.04825.x.
While considerable foodborne pathogen research has been conducted on conventionally produced broilers and turkeys, few studies have focused on free-range (organic) or pastured poultry. The current surveillance study was designed to isolate, identify and genetically characterize Salmonella from pastured poultry farm environment and from retail samples.
In this study, 59 isolates were collected from two pastured poultry farms (n = 164; pens, feed, water and insect traps) and retail carcasses (n = 36) from a local natural foods store and a local processing plant. All isolates were serotyped and analysed phenotypically (antimicrobial resistance profiles) and genotypically (DNA fingerprints, plasmid profiles and integron analysis). Salmonella enterica was detected using standard microbiological methods. Salmonella Kentucky was the most prevalent serotype detected from the sampled sources (53%), followed by Salmonella Enteritidis (24%), Bareilly (10%), Mbandaka (7%), Montevideo (5%) or Newport (2%). All isolates were resistant to sulfisoxazole and novobiocin, and the majority (40/59) possessed class I integrons shown by PCR detection. Each Salmonella serotype elicited a distinct pulsed-field gel electrophoresis fingerprint profile, and unique differences were observed among the serotypes.
The findings of this study show that Salmonella serotypes isolated from pasture-raised poultry exhibit antimicrobial resistance and class I integrons.
This study demonstrates that despite the cessation of antibiotic usage in poultry production, antibiotic resistant Salmonella may still be recovered from the environment and poultry products.
虽然已经对传统生产的肉鸡和火鸡进行了大量的食源性致病菌研究，但很少有研究关注放养（有机）或放牧家禽。本研究旨在从放牧家禽养殖场环境和零售样本中分离、鉴定和遗传特征分析沙门氏菌。
在这项研究中，从两个放牧家禽养殖场（n = 164；鸡舍、饲料、水和昆虫陷阱）和当地天然食品商店和当地加工厂的零售禽体（n = 36）中收集了 59 株分离株。所有分离株均进行血清型鉴定，并进行表型（抗生素耐药谱）和基因型（DNA 指纹图谱、质粒图谱和整合子分析）分析。使用标准微生物学方法检测沙门氏菌。从采样来源中检测到最常见的血清型是肠炎沙门氏菌（53%），其次是肠炎沙门氏菌（24%）、巴雷利沙门氏菌（10%）、姆班达卡沙门氏菌（7%）、蒙得维的亚沙门氏菌（5%）或纽波特沙门氏菌（2%）。所有分离株均对磺胺二甲嘧啶和新生霉素耐药，大多数（40/59）通过 PCR 检测携带 I 类整合子。每个沙门氏菌血清型都引起了独特的脉冲场凝胶电泳指纹图谱，并且在血清型之间观察到了独特的差异。
本研究结果表明，从放牧家禽中分离出的沙门氏菌血清型表现出抗生素耐药性和 I 类整合子。
本研究表明，尽管停止了家禽生产中的抗生素使用，但仍可能从环境和禽产品中回收抗生素耐药的沙门氏菌。
