Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Int J Med Microbiol. 2011 Jan;301(1):64-8. doi: 10.1016/j.ijmm.2010.06.002. Epub 2010 Aug 21.
Early detection of outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) and initiation of adequate infection control measures are important objectives in hospital hygiene. To reach these goals, prompt determination of epidemiologic relatedness of clinical MRSA isolates is essential. Genetic typing methods like pulsed-field gel electrophoresis (PFGE), spa typing, or multilocus sequence typing (MLST) have a high discriminatory power, however, these methods are time consuming and cost intensive. The aim of this study was to investigate the potential of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) for discrimination of major MRSA lineages. By analysis of mass spectra from 25 representative MRSA isolates belonging to the 5 major hospital-acquired (HA) MRSA clonal complexes (CC5, CC8, CC22, CC30, CC45; deduced from spa typing), reproducible spectrum differences were observed at 13 characteristic m/z values allowing robust discrimination of the clonal complexes. When 60 independent clinical MRSA isolates were tested for the presence or absence of the 13 characteristic MALDI-TOF MS peaks, 15 different profiles (MALDI types) could be detected. Hierarchical clustering of the MALDI types showed high concordance with the clonal complexes. Our results suggest that MALDI-TOF MS has the potential to become a valuable first-line tool for inexpensive and rapid typing of MRSA in infection control.
早期发现耐甲氧西林金黄色葡萄球菌（MRSA）的爆发，并启动适当的感染控制措施，是医院卫生的重要目标。为了实现这些目标，及时确定临床耐甲氧西林金黄色葡萄球菌分离株的流行病学相关性至关重要。遗传分型方法，如脉冲场凝胶电泳（PFGE）、spa 分型或多位点序列分型（MLST）具有很高的分辨能力，然而，这些方法既耗时又昂贵。本研究旨在探讨基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）在区分主要耐甲氧西林金黄色葡萄球菌谱系方面的潜力。通过分析 25 株代表性耐甲氧西林金黄色葡萄球菌分离株的质谱，这些分离株属于 5 种主要医院获得性（HA）耐甲氧西林金黄色葡萄球菌克隆复合体（CC5、CC8、CC22、CC30、CC45；根据 spa 分型推断），在 13 个特征 m/z 值处观察到可重复的谱差异，从而能够稳健地区分克隆复合体。当对 60 株独立的临床耐甲氧西林金黄色葡萄球菌分离株进行 13 个特征 MALDI-TOF MS 峰的存在或缺失检测时，可检测到 15 种不同的谱型（MALDI 型）。MALDI 型的层次聚类与克隆复合体高度一致。我们的结果表明，MALDI-TOF MS 有可能成为一种具有成本效益且快速的感染控制中耐甲氧西林金黄色葡萄球菌分型的有价值的一线工具。
