POPCAD Research Group, Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Medellín, Colombia.
Arch Oral Biol. 2010 Oct;55(10):728-36. doi: 10.1016/j.archoralbio.2010.07.008. Epub 2010 Aug 21.
This study aimed to determine whether the variations in the occurrence of gram-negative enteric rods as detected through a longitudinal follow up with three consecutive visits, may be associated with the periodontal clinical status.
Clinical and demographic parameters from 63 untreated chronic periodontitis (CP) subjects and 45 healthy controls (HC) were analyzed. Subgingival plaque samples were obtained from 6 sites in each subject at baseline, 1-week, and 1-month visits and processed using culture and biochemical tests. Culture findings were categorized taking into account the detection frequency of gram-negative enteric rods as persistent presence, transient presence, or absence of enteric rods in any sampling time.
Although transient presence of gram-negative enteric rods was more prevalent in CP subjects (16.7%) than HC subjects (9.3%), the difference was not significant (P>0.05). The majority of subjects showed a transient presence of gram-negative enteric rods at concentrations <2 x 10(2)CFU/mL. Persistent presence of gram-negative enteric rods was not observed in any subject through the entire study period. Moreover, differences in both the detection frequencies of individual species and the total number of gram-negative enteric rods were not statistically significant neither inter- nor intragroups.
The findings of the present study could indicate that gram-negative enteric rods are merely transient microorganisms within the subgingival environment and suggest that the periodontal clinical status appeared not to be influenced by the presence of these species.
本研究旨在确定通过连续三次随访检测到的革兰氏阴性肠杆菌的发生变化是否与牙周临床状况有关。
分析了 63 名未经治疗的慢性牙周炎（CP）患者和 45 名健康对照组（HC）的临床和人口统计学参数。在基线、1 周和 1 个月访视时，从每位受试者的 6 个部位采集龈下菌斑样本，并使用培养和生化试验进行处理。将培养结果考虑到革兰氏阴性肠杆菌的检测频率分为持续存在、短暂存在或任何采样时间均不存在肠杆菌。
尽管 CP 组（16.7%）中革兰氏阴性肠杆菌的短暂存在更为普遍，但与 HC 组（9.3%）相比差异无统计学意义（P>0.05）。大多数受试者在浓度<2 x 10(2)CFU/mL 时表现出革兰氏阴性肠杆菌的短暂存在。在整个研究期间，任何受试者均未观察到革兰氏阴性肠杆菌的持续存在。此外，个体物种和革兰氏阴性肠杆菌总数的检测频率差异在组间和组内均无统计学意义。
本研究的结果表明，革兰氏阴性肠杆菌仅是龈下环境中的短暂微生物，并且牙周临床状况似乎不受这些物种存在的影响。
