Mdegela Robinson H, Laurence Kibona, Jacob Petro, Nonga Hezron Emmanuel
Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, P.O. Box 3021, Morogoro, Tanzania.
Trop Anim Health Prod. 2011 Jan;43(1):83-7. doi: 10.1007/s11250-010-9657-4. Epub 2010 Aug 21.
Occurrences of thermophlic Campylobacter in pigs and pig carcasses was investigated in a cross-sectional study that was carried out in three selected slaughter slabs in Morogoro municipality, Tanzania. Before sampling, the slab hygiene, slaughter, carcass dressing, and meat handling was assessed. Fecal samples were collected from 66 slaughter pigs at the kill floor. After slaughter, a 100-cm(2) area on medial surface of the thigh muscles of dressed carcasses was sampled using sterile cotton swabs. Thereafter, the jejunal, cecal, and colon contents were also sampled. The samples were subjected to standard bacteriological examination using Skirrows protocol. In all slaughter slabs visited, it was found that pig slaughter, dressing, and meat handling was done on the ground under unhygienic condition. All the slaughter slab environment were dirty and had neither tap water or drainage systems. Thermophilic Campylobacter prevalence in slaughtered pig was 66.7% while contamination rate of dressed carcasses was 10.6%. Of the Campylobacter-positive carcasses, five (12.2%) were from the animals which were also positive to Campylobacter. The isolation rate of Campylobacter in the cecum was higher (34.8%) compared to the small intestines (28.8%) and colon (16.7%) although the difference was not statistically significant (P > 0.05). Campylobacter jejuni was the most prevalent species as it constituted 74% of all isolates, while Campylobacter coli was isolated at 26%. This suggests possible risks of infection to people through consumption of contaminated pork or through contact with infected pigs. Cecum was found to be the major part of intestine highly colonized by Campylobacter.
在坦桑尼亚莫罗戈罗市三个选定的屠宰场进行的一项横断面研究中，对猪和猪胴体中的嗜热弯曲杆菌感染情况进行了调查。在采样前，对屠宰场的卫生、屠宰、胴体修整和肉类处理情况进行了评估。在屠宰场的屠宰区从66头待宰猪采集粪便样本。屠宰后，用无菌棉拭子在去毛后的胴体大腿内侧肌肉表面100平方厘米区域进行采样。此后，还对空肠、盲肠和结肠内容物进行了采样。样本采用斯基罗氏培养基进行标准细菌学检查。在所走访的所有屠宰场中，发现猪的屠宰、修整和肉类处理都是在不卫生的地面上进行的。所有屠宰场环境都很脏，既没有自来水也没有排水系统。屠宰猪中嗜热弯曲杆菌的患病率为66.7%，而去毛后胴体的污染率为10.6%。在弯曲杆菌呈阳性的胴体中，有5头（12.2%）来自动物本身弯曲杆菌也呈阳性的猪。弯曲杆菌在盲肠中的分离率（34.8%）高于小肠（28.8%）和结肠（16.7%），尽管差异无统计学意义（P>0.05）。空肠弯曲杆菌是最常见的菌种，占所有分离菌株的74%，而结肠弯曲杆菌的分离率为26%。这表明通过食用受污染猪肉或接触感染猪，人们可能面临感染风险。盲肠被发现是弯曲杆菌高度定植的肠道主要部位。
