Diamandis E P
Department of Clinical Biochemistry, Toronto Western Hospital, Ontario, Canada.
Clin Chim Acta. 1990 Dec 17;194(1):19-50. doi: 10.1016/0009-8981(90)90302-9.
Immunoassay is an established technique which has contributed enormously to biomedical analysis. DNA hybridization (DNA probing) methodology is emerging as the most promising new discipline of laboratory medicine with potential applications in areas such as genetics, pathology, microbiology and oncology. In this review, immunoassay and DNA probing methodologies are considered together because of their many similarities in assay design and labeling systems. Selected labeling systems are described in this paper in order to stress strategies, general principles and future trends. Special attention has been given to systems that introduce linear or exponential amplification. In the author's view, such systems will dominate in future applications. It is anticipated that during the next decade, immunoassay and DNA probing assays will be carried out on completely automated systems.
免疫测定是一种成熟的技术，对生物医学分析做出了巨大贡献。DNA杂交（DNA探针）方法正成为检验医学中最具前景的新学科，在遗传学、病理学、微生物学和肿瘤学等领域具有潜在应用价值。在本综述中，免疫测定和DNA探针方法被一同探讨，因为它们在测定设计和标记系统方面有许多相似之处。本文描述了选定的标记系统，以强调策略、一般原则和未来趋势。特别关注了引入线性或指数扩增的系统。作者认为，此类系统将在未来应用中占据主导地位。预计在未来十年内，免疫测定和DNA探针测定将在完全自动化的系统上进行。
