Cookson P, Sutherland J, Turner C, Bashir S, Wiltshire M, Hancock V, Smith K, Cardigan R
Components Development Laboratory, NHS Blood and Transplant, Cresent Drive, Brentwood, Essex CM15 8DP, UK.
Transfus Med. 2010 Dec;20(6):392-402. doi: 10.1111/j.1365-3148.2010.01034.x. Epub 2010 Aug 26.
Several studies suggest that apoptosis of platelets occurs during storage of platelet concentrates (PC). We sought to determine whether storage of PC in additive solution alters levels of apoptosis during storage beyond the current shelf life (5-7 days).
Pooled buffy coat PC (n = 7) were prepared in either 100% plasma or 70% Composol and stored at 22 °C for 12 days. A third arm of the study stored PC in 100% plasma at 37 °C, which is thought to induce apoptosis. PC were tested for mitochrondrial membrane potential, annexin V binding, microparticles, caspase-3/7 activity and decoy cell death receptor 2, as well as standard platelet quality tests.
Composol units remained ≥pH 6·88, with 36% lower lactate and higher pH vs plasma by day 12 (P < 0·001). Platelet function was better maintained, and activation and apoptotic markers tended to be lower in Composol units towards the end of storage. However, levels of all apoptosis markers assessed were not significantly different in units stored in Composol. Storage at 37 °C saw stronger correlation of apoptotic markers with standard quality tests compared to 22 °C, but loss of correlation of caspase-3/7 activity with other apoptosis markers.
We conclude that storage of platelets in 70% Composol vs 100% plasma does not increase the rate of platelet apoptosis. Our data agree with other studies suggesting that platelet apoptosis is sequential to high levels of activation, but share a significant degree of overlap.
多项研究表明，血小板浓缩物（PC）储存期间会发生血小板凋亡。我们试图确定在添加剂溶液中储存PC是否会在超过当前保质期（5 - 7天）的储存期间改变凋亡水平。
将混合的富血小板血浆PC（n = 7）分别制备于100%血浆或70%Composol中，并在22°C下储存12天。研究的第三组将PC在37°C下于100%血浆中储存，这被认为会诱导凋亡。对PC进行线粒体膜电位、膜联蛋白V结合、微粒、半胱天冬酶 - 3/7活性和诱饵细胞死亡受体2的检测，以及标准血小板质量检测。
到第12天，Composol组的pH值保持≥6·88，乳酸含量比血浆组低36%，pH值更高（P < 0·001）。在储存末期，Composol组的血小板功能得到更好维持，激活和凋亡标志物往往更低。然而，在Composol中储存的单位中，所有评估的凋亡标志物水平并无显著差异。与22°C相比，37°C储存时凋亡标志物与标准质量检测的相关性更强，但半胱天冬酶 - 3/7活性与其他凋亡标志物的相关性丧失。
我们得出结论，70%Composol与100%血浆中储存血小板不会增加血小板凋亡率。我们的数据与其他研究一致，表明血小板凋亡是高水平激活后的后续过程，但存在显著程度的重叠。
