Orholm M, Holten-Andersen W, Lundgren J D
Department of Infectious Diseases, Hvidovre Hospital, University of Copenhagen, Denmark.
Eur J Clin Microbiol Infect Dis. 1990 Dec;9(12):880-5. doi: 10.1007/BF01967503.
To assess whether a recently developed indirect immunofluorescent stain using monoclonal antibodies was more sensitive in detecting Pneumocystis carinii than the combination of Giemsa and methenamine silver nitrate stains which has routinely been used in the laboratory, 88 lavage fluid specimens and 34 induced sputum specimens were examined. All specimens were stained by five techniques: immunofluorescence using a combination of three monoclonal antibodies (from the National Institutes of Health, USA), immunofluorescence using a single monoclonal antibody (from Dakopatts), Giemsa, methenamine silver nitrate and toluidine blue O. Immunofluorescence using the monoclonal antibodies from the NIH was significantly more sensitive than any other single staining method and than the combination of Giemsa and methenamine silver nitrate staining. The study also showed that the cytospin centrifuge was very suitable for the preparation of slides with lavage fluid and processed induced sputum. Finally, the sensitivity of examination of induced sputum to detect Pneumocystis carinii was found to be 50% when compared with bronchoalveolar lavage fluid. However, this sensitivity may increase through practice.
为评估一种最近研发的使用单克隆抗体的间接免疫荧光染色法在检测卡氏肺孢子虫方面是否比实验室常规使用的吉姆萨染色法和亚甲胺银染色法联合使用更敏感，对88份灌洗液标本和34份诱导痰标本进行了检查。所有标本均采用五种技术染色：使用三种单克隆抗体组合（来自美国国立卫生研究院）的免疫荧光法、使用一种单克隆抗体（来自达科帕茨公司）的免疫荧光法、吉姆萨染色法、亚甲胺银染色法和甲苯胺蓝O染色法。使用来自美国国立卫生研究院的单克隆抗体的免疫荧光法比任何其他单一染色方法以及吉姆萨染色法和亚甲胺银染色法联合使用都显著更敏感。该研究还表明，细胞离心涂片法非常适合用于制备灌洗液和处理后的诱导痰的玻片。最后，发现与支气管肺泡灌洗液相比，诱导痰检测卡氏肺孢子虫的敏感性为50%。然而，通过实践这种敏感性可能会提高。
