Pawelec G, Brons G
Clin Exp Immunol. 1978 Mar;31(3):426-35.
The effects of the immunosuppressive sulphated polygalactan lambda carrageenan on in vitro models of allograft immunity were compared with the effects of removing macrophages (surface adherent and/or phagocytic cells) by established methods. Carrageenan depressed the primary mixed lymphocyte reactions, but not to the same extent as the removal of macrophages. 2-Mercaptoethanol restored the response. Secondary mixed lymphocyte reactions and responses to phytohaemaglutinin were depressed by carrageenan but not by the removal of macrophages, and in these systems 2-mercaptoethanol failed to restore the responses of carrageenan-treated cultures. In contrast, cell-mediated cytolysis by presensitized lymphocytes was not affected by carrageenan or by colloidal silica. Carrageenan depressed cell-mediated cytolysis only if it was present during the sensitization of the effector cells. We conclude that carrageenan can have two dose-related effects in vitro: one on the macrophage and one on the responding lymphocyte.
将免疫抑制性硫酸化聚半乳糖λ-角叉菜胶对同种异体移植免疫体外模型的作用，与通过既定方法去除巨噬细胞（表面黏附细胞和/或吞噬细胞）的作用进行了比较。角叉菜胶抑制初次混合淋巴细胞反应，但程度不及去除巨噬细胞。2-巯基乙醇可恢复反应。角叉菜胶可抑制二次混合淋巴细胞反应和对植物血凝素的反应，但去除巨噬细胞则无此作用，并且在这些系统中，2-巯基乙醇无法恢复经角叉菜胶处理的培养物的反应。相比之下，预先致敏淋巴细胞介导的细胞溶解不受角叉菜胶或胶体二氧化硅的影响。角叉菜胶仅在效应细胞致敏期间存在时才会抑制细胞介导的细胞溶解。我们得出结论，角叉菜胶在体外可产生两种与剂量相关的作用：一种作用于巨噬细胞，另一种作用于反应性淋巴细胞。
