Takematsu H, Isono N, Kato T, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sendai.
Tohoku J Exp Med. 1990 Sep;162(1):1-13. doi: 10.1620/tjem.162.1.
Human epidermal keratinocytes constitutively produce a variety of cytokines, including neutrophil chemotactic peptide named epidermal cell-derived thymocyte-activating factor, which has been later confirmed to be interleukin 1 (IL-1). Because recombinant IL-1 lacks chemotactic activity, in the present study, we examined the exact nature of the neutrophil chemotactic peptide in the culture supernatant of normal human epidermal keratinocytes. Normal human epidermal keratinocytes produced a neutrophil chemotactic factor, which was also chemotactic for T lymphocytes. Molecular sieve chromatography revealed an approximate molecular size of 11,000 daltons. The activity was retained after heating at 100 degrees C for 10 min, and at a pH between 4 and 11, but was partially inactivated at pH 3, or by trypsin treatment. The chemotactic activity was not inhibited by the treatment with anti-IL-1 antibody. Its production by keratinocytes was stimulated by IL-1 and lipopolysaccharide but not by UV irradiation, tumor necrosis factor-alpha or by interferon-gamma. The neutrophil chemotactic activity in vivo was confirmed by the intradermal injection of the factor into guinea pigs. Blocking study with monoclonal antibodies against NAP-1/IL-8 confirmed that the neutrophil chemotactic factor is IL-8.
人表皮角质形成细胞可组成性地产生多种细胞因子，包括一种名为表皮细胞衍生的胸腺细胞激活因子的中性粒细胞趋化肽，后来证实其为白细胞介素1（IL-1）。由于重组IL-1缺乏趋化活性，在本研究中，我们检测了正常人表皮角质形成细胞培养上清液中中性粒细胞趋化肽的确切性质。正常人表皮角质形成细胞产生一种中性粒细胞趋化因子，它对T淋巴细胞也有趋化作用。分子筛层析显示其近似分子量为11,000道尔顿。该活性在100℃加热10分钟以及在pH值为4至11时仍能保留，但在pH值为3时或经胰蛋白酶处理后会部分失活。抗IL-1抗体处理不会抑制其趋化活性。角质形成细胞对其产生可被IL-1和脂多糖刺激，但不受紫外线照射、肿瘤坏死因子-α或干扰素-γ刺激。通过将该因子皮内注射到豚鼠体内，证实了其在体内的中性粒细胞趋化活性。用抗NAP-1/IL-8单克隆抗体进行的阻断研究证实，中性粒细胞趋化因子为IL-8。
