Morita E, Yamada S, Kimura I, Nakamura K, Sugita Y, Yamamoto S
Department of Dermatology, Hiroshima University School of Medicine, Japan.
Skin Pharmacol. 1993;6(3):161-9. doi: 10.1159/000211129.
There is much evidence to support the theory that keratinocytes and dermal fibroblasts actively participate in inflammatory reactions by the production of proinflammatory mediators or cytokines. We investigated the neutrophil chemotactic activity in conditioned media of cultured epidermal cells and dermal fibroblasts, and found that an epidermal cell-derived factor induced fibroblasts to produce a neutrophil chemotactic factor. This neutrophil chemotactic factor was identified as interleukin-8 (IL-8) by the elution position on HPLC and by a neutralization test that uses monoclonal anti-IL-8 antibody (14E4). The epidermal cell-derived factor was fractionated together with thymocyte-proliferating activity on Sephadex G-75 gel chromatography followed by HPLC. It was blocked specifically by anti-interleukin-1 (IL-1) alpha antibody, which indicates that this factor was IL-1 alpha. Since a variety of inflammatory dermatoses is characterized by the infiltration of neutrophils into the skin, induction of IL-8 production in fibroblasts by epidermal cells may play an important role in inflammatory skin diseases.
有许多证据支持角质形成细胞和真皮成纤维细胞通过产生促炎介质或细胞因子而积极参与炎症反应这一理论。我们研究了培养的表皮细胞和真皮成纤维细胞条件培养基中的中性粒细胞趋化活性，发现一种表皮细胞衍生因子可诱导成纤维细胞产生中性粒细胞趋化因子。通过高效液相色谱（HPLC）上的洗脱位置以及使用单克隆抗白细胞介素-8（IL-8）抗体（14E4）的中和试验，将这种中性粒细胞趋化因子鉴定为白细胞介素-8（IL-8）。表皮细胞衍生因子在Sephadex G-75凝胶色谱上与胸腺细胞增殖活性一起分级分离，随后进行HPLC分析。它被抗白细胞介素-1（IL-1）α抗体特异性阻断，这表明该因子是IL-1α。由于多种炎症性皮肤病的特征是中性粒细胞浸润到皮肤中，表皮细胞诱导成纤维细胞产生IL-8可能在炎症性皮肤病中起重要作用。
