Jeju National University Biotechnology Regional Innovation Center, Jeju, Republic of Korea.
J Microbiol. 2010 Aug;48(4):433-7. doi: 10.1007/s12275-010-0088-8. Epub 2010 Aug 20.
A strictly aerobic, red-pigmented, non-motile, catalase- and oxidase-positive, Gram-staining-negative bacterium, designated strain CNURIC011(T), was isolated from seawater off the coast of Jeju Island in Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNURIC011(T) belongs to the genus Aquimarina in the family Flavobacteriaceae. 16S rRNA gene sequence analysis revealed that the close relatives of the novel strain are Aquimarina latercula ATCC 23177(T), Aquimarina marcrocephali JAMB N27(T), Aquimarina intermedia KMM 6258(T), Aquimarina muelleri KMM 6020(T), and Aquimarina brevivitae SMK-19(T), with sequence similarities of 97.6, 96.6, 96.0, 95.6, and 94.2%, respectively. DNA-DNA hybridization revealed that the level of relatedness between strain CNURIC011(T) and Aquimarina latercula ATCC 23177(T) (=KCTC 2912(T)) was 4.9%. The DNA G+C content was 35.8 mol% and the major respiratory quinone was MK-6. The major fatty acids were iso-C(15:0) (14.9%), C(15:0) (13.9%), iso-C(17:0) 3-OH (12.6%), iso-C(15:1) G (7.3%), and iso-C(17:1) omega9c (7.2%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC011(T) represents a novel species within the genus Aquimarina, for which the name Aquimarina litoralis sp. nov. is proposed. The type strain is CNURIC011(T) (=KCTC 22614(T) =JCM 15974(T)).
一株严格需氧、红色、非运动、过氧化氢酶和氧化酶阳性、革兰氏染色阴性的细菌，被命名为 Aquimarina litoralis 株 CNURIC011(T)，从韩国济州岛沿海的海水中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CNURIC011(T)属于黄杆菌科的 Aquimarina 属。16S rRNA 基因序列分析显示，该新菌株的近亲是 Aquimarina latercula ATCC 23177(T)、Aquimarina marcrocephali JAMB N27(T)、Aquimarina intermedia KMM 6258(T)、Aquimarina muelleri KMM 6020(T)和 Aquimarina brevivitae SMK-19(T)，其序列相似度分别为 97.6%、96.6%、96.0%、95.6%和 94.2%。DNA-DNA 杂交显示，菌株 CNURIC011(T)与 Aquimarina latercula ATCC 23177(T)（=KCTC 2912(T)）的相关性为 4.9%。DNA G+C 含量为 35.8 mol%，主要呼吸醌为 MK-6。主要脂肪酸为 iso-C(15:0)（14.9%）、C(15:0)（13.9%）、iso-C(17:0) 3-OH（12.6%）、iso-C(15:1) G（7.3%）和 iso-C(17:1) omega9c（7.2%）。基于表型、系统发育和基因型数据，菌株 CNURIC011(T)代表 Aquimarina 属中的一个新种，命名为 Aquimarina litoralis sp. nov.。模式菌株为 CNURIC011(T)（=KCTC 22614(T) =JCM 15974(T)）。
