Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong 226001, China.
Colloids Surf B Biointerfaces. 2010 Dec 1;81(2):406-11. doi: 10.1016/j.colsurfb.2010.06.019. Epub 2010 Jul 6.
34 nm gold nanoparticles with good stability were synthesized and characterized and their effect (as a function of concentration) on the proliferation of keratinocytes was evaluated by means of MTT and nucleolar organizer region (AgNOR) count (silver staining). The cell morphology was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results demonstrate that a low concentration of gold nanoparticles enhances the proliferation of keratinocytes. Specifically, a concentration of 5.0 ppm gold nanoparticle has the best effect on the promotion of cell growth. In the experiment group, the AgNOR-positive areas and AgNOR area/nuclear area ratios of keratinocytes co-cultured with 5.0 ppm gold nanoparticles were greater than those in the control group (p<0.01). At a level greater than 10.0 ppm, gold nanoparticles were found to have a cytotoxic effect on keratinocytes. It is concluded that a low concentration of gold nanoparticles may be used as a biomedical material in skin tissue engineering.
合成并表征了粒径为 34nm 的金纳米粒子，稳定性良好，通过 MTT 和核仁组成区（AgNOR）计数（银染）评估其对角质形成细胞增殖的影响（作为浓度的函数）。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察细胞形态。结果表明，低浓度的金纳米粒子可增强角质形成细胞的增殖。具体而言，金纳米粒子浓度为 5.0ppm 时对细胞生长的促进作用最佳。在实验组中，与 5.0ppm 金纳米粒子共培养的角质形成细胞的 AgNOR 阳性区域和 AgNOR 面积/核面积比值大于对照组（p<0.01）。浓度大于 10.0ppm 时，金纳米粒子对角质形成细胞具有细胞毒性作用。结论：低浓度的金纳米粒子可用作皮肤组织工程中的生物医学材料。
