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疱疹病毒六邻体衣壳粒六重对称性的直接证据。

Direct evidence for 6-fold symmetry of the herpesvirus hexon capsomere.

作者信息

Furlong D

出版信息

Proc Natl Acad Sci U S A. 1978 Jun;75(6):2764-6. doi: 10.1073/pnas.75.6.2764.

DOI:10.1073/pnas.75.6.2764
PMID:208076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392644/
Abstract

Rotational power spectrum analysis of scanning transmission electron micrographs of negatively stained herpesvirus capsids has given direct evidence for the 6-fold symmetry of the herpesvirus hexon capsomere. Individual hexons have been analyzed in situ without interference from other parts of the capsid because only one level of the capsid was in focus in the micrograph. This study found hexons to have 6-fold symmetry. The majority of the mass of each hexon subunit was seen to lie on a line connecting the centers of adjacent capsomeres. This agrees with analysis of conventional micrographs of capsid fragments, which are also presented, as well as with biochemical data and theoretical expectations.

摘要

对负染疱疹病毒衣壳的扫描透射电子显微照片进行旋转功率谱分析，为疱疹病毒六邻体壳粒的六重对称性提供了直接证据。由于在显微照片中只有衣壳的一个层面处于聚焦状态，因此对单个六邻体进行了原位分析，不受衣壳其他部分的干扰。本研究发现六邻体具有六重对称性。每个六邻体亚基的大部分质量位于连接相邻壳粒中心的一条直线上。这与同时展示的衣壳片段传统显微照片分析结果一致，也与生化数据和理论预期相符。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b27c/392644/f5060484bfc0/pnas00018-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b27c/392644/369f0f68467a/pnas00018-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b27c/392644/f5060484bfc0/pnas00018-0231-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b27c/392644/369f0f68467a/pnas00018-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b27c/392644/f5060484bfc0/pnas00018-0231-a.jpg

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Physical principles in the construction of regular viruses.常规病毒构建中的物理原理。
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