Fujita Akira, Ajisawa Atsushi, Harada Nobuyuki, Higuchi Kazue, Mori Toru
Department of Pulmonary Medicine, Tokyo Metropolitan Tama Medical Center, 2-8-29 Musashidai, Fuchu-shi, Tokyo 183-8524, Japan.
Clin Dev Immunol. 2011;2011. doi: 10.1155/2011/325295. Epub 2010 Aug 10.
To evaluate the usefulness of one of IGRAs, QuantiFERON-TB Gold (QFT-G), in human immunodeficiency virus- (HIV-) infected patients with various CD4(+) T cell counts.
The QFT-G assay was performed using QFT-G kits among 107 HIV-infected patients including 9 cases with active tuberculosis (TB).
In HIV-infected patients with CD4(+) > 50/microL, QFT-G positive rate for active TB patients was 5/6 (sensitivity = 83%), and that for those without active disease was 1/69 (specificity = 99%). The frequency of indeterminate QFT-G test was significantly higher in those with CD4(+) less than 50/microL (P < .0001). At the same time there was a proportional relationship between CD4(+) and interferon-gamma response to mitogen (positive control) in QFT-G test (P = .0001). Conclusions. Our data suggested that QFT-G had high sensitivity and specificity in HIV-infected populations with CD4(+) greater than 50/microL. However, QFT-G did not perform well in HIV-positive patients with CD4(+) less than 50/microL.
评估结核感染T细胞检测（IGRAs）之一的全血γ干扰素释放试验（QFT-G）在不同CD4(+) T细胞计数的人类免疫缺陷病毒（HIV）感染患者中的应用价值。
使用QFT-G检测试剂盒对107例HIV感染患者进行QFT-G检测，其中包括9例活动性结核病（TB）患者。
在CD4(+) > 50/μL的HIV感染患者中，活动性TB患者的QFT-G阳性率为5/6（敏感性 = 83%），无活动性疾病患者的阳性率为1/69（特异性 = 99%）。CD4(+)小于50/μL的患者中QFT-G检测不确定的频率显著更高（P < .0001）。同时，QFT-G检测中CD4(+)与对丝裂原（阳性对照）的γ干扰素反应之间存在比例关系（P = .0001）。结论。我们的数据表明，QFT-G在CD4(+)大于50/μL的HIV感染人群中具有高敏感性和特异性。然而，QFT-G在CD4(+)小于50/μL的HIV阳性患者中表现不佳。
