Mazumder Aprotim, Roopa T, Kumar Abhishek, Iyer K Venkatesan, Ramdas Nisha M, Shivashankar G V
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, India.
Methods Cell Biol. 2010;98:221-39. doi: 10.1016/S0091-679X(10)98010-2.
The nucleus is maintained in a prestressed state within eukaryotic cells, stabilized mechanically by chromatin structure and other nuclear components on its inside, and cytoskeletal components on its outside. Nuclear architecture is emerging to be critical to the governance of chromatin assembly, regulation of genome function and cellular homeostasis. Elucidating the prestressed organization of the nucleus is thus important to understand how the nuclear architecture impinges on its function. In this chapter, various chemical and mechanical methods have been described to probe the prestressed organization of the nucleus.
在真核细胞中，细胞核维持在预应力状态，其内部通过染色质结构和其他核成分、外部通过细胞骨架成分实现机械稳定。核结构对于染色质组装的调控、基因组功能的调节以及细胞内稳态的维持正变得至关重要。因此，阐明细胞核的预应力组织对于理解核结构如何影响其功能很重要。在本章中，已描述了多种化学和机械方法来探究细胞核的预应力组织。
