Laboratory of Pleura, Pulmonary Division, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
Int J Tuberc Lung Dis. 2010 Sep;14(9):1153-8.
A tertiary care research centre in São Paolo, Brazil.
To quantify interleukin (IL) 8, tumour necrosis factor alpha (TNF-alpha), vascular endothelial growth factor (VEGF) and transforming growth factor beta(1) (TGF-beta(1)) in pleural fluid from tuberculous patients, correlating its values with the histopathological patterns in pleural biopsies.
Cytokines were quantified in patients with transudates secondary to congestive heart failure (n = 8) and exudates secondary to tuberculosis (TB; n = 39). In parietal pleural biopsies from TB patients, the histological patterns of the inflammatory response were quantified by morphometric analysis (stereological point-counting method).
IL-8, TNF-alpha, VEGF and TGF-beta(1) levels were higher in TB than in transudates. A positive correlation existed between components of the fibrinoid exudative phase with pleural fluid IL-8 (R = 0.52, P = 0.004) and VEGF (R = 0.42, P = 0.0021) levels. A negative correlation existed between pleural fluid IL-8 (R = -0.37, P = 0.048) and VEGF (R = -0.44, P = 0.0015) levels with tissue components of fibroproliferation.
The high pleural levels of TNF-alpha, IL-8, VEGF and TGF-beta(1) suggest the involvement of these cytokines in the TB immunological response. The positive correlation between pleural fluid IL-8 and VEGF with the components of the acute exudative phase and the negative correlation between these cytokines with the fibroproliferative components suggest a temporary inflammatory response in the pleural space.
巴西圣保罗的一家三级保健研究中心。
定量检测胸腔积液中白细胞介素 8（IL-8）、肿瘤坏死因子-α（TNF-α）、血管内皮生长因子（VEGF）和转化生长因子-β1（TGF-β1），并与胸腔活检的组织病理学模式相关联。
对心力衰竭所致胸腔漏出液（n = 8）和结核性胸腔渗出液（TB；n = 39）患者的胸腔积液中的细胞因子进行定量检测。对 TB 患者的壁层胸膜活检组织进行炎症反应组织学模式的定量分析（体视学计点法）。
TB 患者胸腔积液中 IL-8、TNF-α、VEGF 和 TGF-β1 水平均高于漏出液。胸腔积液中纤维蛋白渗出期的各个成分与 IL-8（R = 0.52，P = 0.004）和 VEGF（R = 0.42，P = 0.0021）呈正相关。胸腔积液中 IL-8（R = -0.37，P = 0.048）和 VEGF（R = -0.44，P = 0.0015）与组织纤维增生期成分呈负相关。
胸腔积液中 TNF-α、IL-8、VEGF 和 TGF-β1 水平较高，提示这些细胞因子参与了 TB 的免疫反应。胸腔积液中 IL-8 和 VEGF 与急性渗出期成分呈正相关，与纤维增生期成分呈负相关，提示胸腔内存在短暂的炎症反应。
