Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi, India.
Pharm Biol. 2010 Dec;48(12):1397-404. doi: 10.3109/13880209.2010.486837. Epub 2010 Sep 7.
Withania coagulans Dunal. (Solanaceae) has been shown to possess hypoglycemic, free radical scavenging and wound healing activity. Therefore, it may be worthwhile to study the effect of Withania coagulans in diabetic wound healing.
This study included determination of rate of wound contraction and estimation of various biochemical parameters such as collagen, hexosamine, total protein, total DNA, SOD and CAT levels in the granulation tissues.
The hydroalcoholic fraction of the methanolic extract (standardized by withaferin-A using high performance thin layer chromatography (HPTLC) )of Withania coagulans in the form of 10% w/w ointment topically and at a dose of 500 mg/kg per oral (p.o.) was administered to streptozotocin-induced diabetic rats. The results obtained were compared with diabetic control and Aloe barbadensis Miller (syn. Aloe vera L.) (Liliaceae) was used as a reference drug.
The amount of withaferin-A present in the methanolic extract was 3.67 mg/g of the extract. The hydroalcoholic fraction in both the forms, i.e., topical (10% w/w ointment) and oral (500 mg/kg, p.o.) showed a significant increase in the rate of wound contraction (83.02% topical and 65.14% oral) topical and 93.18% oral) when compared to diabetic control (66 which was further justified with significant increase in the levels of collagen, protein, DNA, SOD, CAT, and decreased level of hexosamine.
The hydroalcoholic fraction of Withania coagulans in oral form is found to be more effective than the topical form. However, further studies are in progress to find the lead molecules responsible for the wound healing effect.
已证实 Withania coagulans Dunal.（茄科）具有降血糖、清除自由基和促进伤口愈合的活性。因此，研究 Withania coagulans 对糖尿病伤口愈合的影响可能是值得的。
本研究包括测定伤口收缩率，并评估肉芽组织中的各种生化参数，如胶原蛋白、己糖胺、总蛋白、总 DNA、SOD 和 CAT 水平。
以 10%重量/重量的软膏形式局部给予和以 500mg/kg 口服（p.o.）的方式给予 Withania coagulans 的甲醇提取物的水醇级分（用高效薄层色谱法（HPTLC）用 withaferin-A 标准化），制成糖尿病大鼠的 10%重量/重量软膏，制成糖尿病大鼠的 10%重量/重量软膏，制成糖尿病大鼠的 10%重量/重量软膏。将所得结果与糖尿病对照组进行比较，并将 Aloe barbadensis Miller（同义词 Aloe vera L.）（百合科）用作参考药物。
甲醇提取物中存在的 withaferin-A 量为 3.67mg/g 提取物。两种形式（局部[10%重量/重量软膏]和口服[500mg/kg，p.o.）的水醇级分均显示出伤口收缩率的显著增加（局部 83.02%和口服 65.14%），与糖尿病对照组（66）相比，这进一步证明了胶原蛋白、蛋白质、DNA、SOD、CAT 的水平显著增加，己糖胺的水平降低。
口服形式的 Withania coagulans 的水醇级分被发现比局部形式更有效。然而，正在进行进一步的研究，以找到负责伤口愈合作用的先导分子。
