College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Colloids Surf B Biointerfaces. 2011 Jan 1;82(1):1-7. doi: 10.1016/j.colsurfb.2010.07.043. Epub 2010 Jul 30.
Biodegradable hydrogels are important biomaterials for tissue engineering and drug delivery. For the purpose of corneal regenerative medicine, we describe an in situ formed hydrogel based on a water-soluble derivative of chitosan, hydroxypropyl chitosan (HPCTS), and sodium alginate dialdehyde (SAD). Periodate oxidized alginate rapidly cross-links HPCTS due to Schiff's base formation between the available aldehyde and amino groups. Hydrogel cytotoxicity, degradability and histocompatibility in vivo were examined. The potential of the composite hydrogel for corneal endothelium reconstruction was demonstrated by encapsulating corneal endothelial cells (CECs) to grow on Descemet's membranes. The results demonstrate that the composite hydrogel was both non-toxic and biodegradable and that CECs transplanted by the composite hydrogel could survive and retain normal morphology. These results provide an opportunity for corneal endothelium reconstruction based on tissue engineering by the in situ formed composite hydrogel.
可生物降解水凝胶是组织工程和药物输送的重要生物材料。为了实现角膜再生医学的目的，我们描述了一种基于壳聚糖水溶性衍生物羟丙基壳聚糖（HPCTS）和醛化海藻酸钠（SAD）的原位形成水凝胶。高碘酸盐氧化的海藻酸钠由于可用醛基和氨基之间的希夫碱形成而快速交联 HPCTS。体内研究了水凝胶的细胞毒性、可降解性和组织相容性。通过将角膜内皮细胞（CECs）包封在 Descemet 膜上，证明了复合水凝胶用于角膜内皮重建的潜力。结果表明，复合水凝胶既无毒又可生物降解，并且复合水凝胶移植的 CECs 可以存活并保持正常形态。这些结果为基于组织工程的角膜内皮重建提供了机会，方法是通过原位形成的复合水凝胶。
