Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Plant Physiol Biochem. 2010 Oct-Nov;48(10-11):903-8. doi: 10.1016/j.plaphy.2010.08.003. Epub 2010 Aug 19.
Changes in antioxidant systems in soybean [Glycine max (L.) Merr., Fabaceae] genotypes infected with Sclerotinia sclerotiorum were studied 12, 24, 48 and 72h after inoculation. Generation of superoxide and hydroxyl radicals was evaluated together with the production of malonyldialdehyde, main end product of lipid peroxidation. Several enzymatic and non-enzymatic parameters were monitored as well, such as the activity of antioxidant enzymes superoxide dismutase and pyrogallol and guaiacol peroxidases, reduced glutathione, soluble proteins and total carotenoids content. Results showed that genotypes expressed oxidative burst as well as different antioxidant systems in response to biotic stress caused by pathogen invasion. It has been confirmed that, although hypersensitive cell death is efficient against biotrophic pathogens, it does not protect soybean plants against infection by the necrotrophic pathogen such as S. sclerotiorum. Still, some genotypes showed distinctive and combined activity of several biochemical parameters which may point to further directions in exploring host-pathogen relations and lead to selection and production of new genotypes with higher levels of tolerance.
研究了感染核盘菌（Sclerotinia sclerotiorum）后 12、24、48 和 72 小时大豆（Glycine max（L.）Merr.，豆科）基因型中抗氧化系统的变化。评估了超氧阴离子和羟基自由基的产生，以及脂质过氧化的主要终产物丙二醛的产生。还监测了几种酶促和非酶促参数，如抗氧化酶超氧化物歧化酶和焦儿茶酚、愈创木酚过氧化物酶的活性、还原型谷胱甘肽、可溶性蛋白质和总类胡萝卜素含量。结果表明，基因型对由病原体入侵引起的生物胁迫表现出氧化爆发和不同的抗氧化系统。已经证实，尽管过敏细胞死亡对生物营养病原体有效，但它不能保护大豆植物免受坏死病原体如核盘菌的感染。然而，一些基因型表现出几种生化参数的独特和组合活性，这可能为探索宿主-病原体关系指明进一步的方向，并导致选择和生产具有更高耐受性的新基因型。
