Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung, Taiwan, ROC.
Fish Shellfish Immunol. 2010 Dec;29(6):1092-8. doi: 10.1016/j.fsi.2010.09.002. Epub 2010 Sep 17.
White shrimp Litopenaeus vannamei which had been immersed in seawater (35‰, pH 8.2) containing the hot-water extract of Spirulina platensis at 0 (control), 200, 400, and 600 mg L(-1) for 3 h, were transferred to seawater at pH 6.8, and the immune parameters and transcripts of the lipopolysaccharide- and β-glucan-binding protein (LGBP), peroxinectin (PX), and integrin β (IB) were examined 6-96 h post-transfer. Shrimp with no exposure to the hot-water extract and no pH change served as the background control. Results indicated that the hyaline cells, granular cells (including semi-granular cells), total haemocyte count, phenoloxidase activity, respiratory burst, superoxide dismutase activity, glutathione peroxidase activity, and lysozyme activity of shrimp transferred to seawater at pH 6.8 significantly decreased to the lowest at 6 h post-transfer. These immune parameters of shrimp immersed in 600 mg L(-1) of the extract were significantly higher than those of control shrimp at 24-96 h post-transfer, and had returned to the background values earlier at 48-72 h post-transfer with significant transcripts of LGBP, PX, and IB at 24, 6, and 24 h, respectively, whereas these parameters of control shrimp returned to the original values at ≥96 h post-transfer.
凡纳滨对虾（Litopenaeus vannamei）先在含有螺旋藻（Spirulina platensis）热水提取物（浓度分别为 0（对照）、200、400 和 600 mg/L）的 35‰海水（pH8.2）中浸泡 3 h，然后转移到 pH6.8 的海水中，6-96 h 后检测免疫参数和脂多糖/β-葡聚糖结合蛋白（LGBP）、过氧化物酶（PX）和整合素 β（IB）的转录本。未暴露于热水提取物且未发生 pH 变化的虾作为背景对照。结果表明，转移到 pH6.8 的海水中后，虾的无颗粒细胞、颗粒细胞（包括半颗粒细胞）、总血细胞计数、酚氧化酶活性、呼吸爆发、超氧化物歧化酶活性、谷胱甘肽过氧化物酶活性和溶菌酶活性均显著降低，在 6 h 时达到最低。与对照组相比，浸泡在 600 mg/L 提取物中的虾在 24-96 h 后这些免疫参数显著升高，在 48-72 h 后恢复到背景值，LGBP、PX 和 IB 的转录本分别在 24、6 和 24 h 时显著上调，而对照组虾的这些参数在≥96 h 后恢复到原始值。
