基于镧系元素的荧光探针用于水和活细胞中过氧化氢的选择性时间门控检测。
Lanthanide-based luminescent probes for selective time-gated detection of hydrogen peroxide in water and in living cells.
机构信息
Department of Chemistry and the Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
出版信息
Chem Commun (Camb). 2010 Oct 28;46(40):7510-2. doi: 10.1039/c0cc01560a. Epub 2010 Sep 14.
Lanthanide-based luminescent probes TPR1 and TPR2 were developed for the detection of hydrogen peroxide (H(2)O(2)) in living systems. The chemoselective reaction of these boronate-protected probes with H(2)O(2) resulted in an enhanced lanthanide sensitization and a 6-fold increase in luminescent intensity. TPR2 was utilized to measure the endogenous production of H(2)O(2) in RAW 264.7 macrophages using time-gated luminescent spectroscopy.
研发了基于镧系元素的荧光探针 TPR1 和 TPR2 用于检测活系统中的过氧化氢（H(2)O(2)）。这些硼酸盐保护的探针与 H(2)O(2)的选择性化学反应导致镧系元素敏化增强和发光强度增加 6 倍。使用时间门控荧光光谱法，TPR2 用于测量 RAW 264.7 巨噬细胞中内源性 H(2)O(2)的产生。
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