Department of Chemistry and the Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
Chem Commun (Camb). 2010 Oct 28;46(40):7510-2. doi: 10.1039/c0cc01560a. Epub 2010 Sep 14.
Lanthanide-based luminescent probes TPR1 and TPR2 were developed for the detection of hydrogen peroxide (H(2)O(2)) in living systems. The chemoselective reaction of these boronate-protected probes with H(2)O(2) resulted in an enhanced lanthanide sensitization and a 6-fold increase in luminescent intensity. TPR2 was utilized to measure the endogenous production of H(2)O(2) in RAW 264.7 macrophages using time-gated luminescent spectroscopy.
研发了基于镧系元素的荧光探针 TPR1 和 TPR2 用于检测活系统中的过氧化氢（H(2)O(2)）。这些硼酸盐保护的探针与 H(2)O(2)的选择性化学反应导致镧系元素敏化增强和发光强度增加 6 倍。使用时间门控荧光光谱法，TPR2 用于测量 RAW 264.7 巨噬细胞中内源性 H(2)O(2)的产生。
