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蛹虫草通过诱导 MHC 分子表达和细胞因子产生增强 MHC 限制性抗原呈递。

Cordyceps militaris Enhances MHC-restricted Antigen Presentation via the Induced Expression of MHC Molecules and Production of Cytokines.

机构信息

College of Pharmacy, Sahmyook University, Seoul 139-742, Korea.

出版信息

Immune Netw. 2010 Aug;10(4):135-43. doi: 10.4110/in.2010.10.4.135. Epub 2010 Aug 31.

DOI:10.4110/in.2010.10.4.135
PMID:20844738
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2939358/
Abstract

BACKGROUND

Cordyceps militarys water extract (CME) has been reported to exert antitumor and immunomodulatory activities in vivo and in vitro. However, the therapeutic mechanism has not yet been elucidated. In this study, we examined the effects of CME on the antigen presenting function of antigen presenting cells (APCs).

METHODS

Dendritic cells (DCs) were cultured in the presence of CME, and then allowed to phagocytose microspheres containing ovalbumin (OVA). After washing and fixing the efficacy of OVA, peptide presentation by DCs were evaluated using CD8 and CD4 T cells. Also, we confirmed the protein levels of proinflammatory cytokines through western blot analysis.

RESULTS

CME enhanced both MHC class I and class II-restricted presentation of OVA in DCs. In addition, the expression of both MHC class I and II molecules was enhanced, but there was no changes in the phagocytic activity of exogenous OVA. Furthermore, CME induced the protein levels of iNOS, COX-2, proinflammatory cytokines, and nuclear p65 in a concentration-dependent manner, as determined by western blot.

CONCLUSION

These results provide an understanding of the mechanism of the immuno-enhancing activity of CME on the induction of MHC-restricted antigen presentation in relation to their actions on APCs.

摘要

背景

蛹虫草水提取物（CME）已被报道在体内和体外具有抗肿瘤和免疫调节活性。然而，其治疗机制尚未阐明。在这项研究中，我们研究了 CME 对抗原呈递细胞（APC）抗原呈递功能的影响。

方法

在存在 CME 的情况下培养树突状细胞（DC），然后让其吞噬含有卵清蛋白（OVA）的微球。洗涤和固定 OVA 后，使用 CD8 和 CD4 T 细胞评估 DC 肽呈递。此外，我们通过 Western blot 分析确认了促炎细胞因子的蛋白水平。

结果

CME 增强了 DC 中 MHC I 类和 II 类限制的 OVA 呈递。此外，MHC I 类和 II 类分子的表达均增强，但外源性 OVA 的吞噬活性没有变化。此外，CME 以浓度依赖性方式诱导 iNOS、COX-2、促炎细胞因子和核 p65 的蛋白水平，这通过 Western blot 确定。

结论

这些结果提供了对 CME 对 MHC 限制抗原呈递诱导的免疫增强活性的机制的理解，与它们对 APC 的作用有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/44017435267c/in-10-135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/6158dd15ff0a/in-10-135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/98235739b60c/in-10-135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/36f61f8f77ab/in-10-135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/474eb2b3d31c/in-10-135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/44017435267c/in-10-135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/6158dd15ff0a/in-10-135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/98235739b60c/in-10-135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/36f61f8f77ab/in-10-135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/474eb2b3d31c/in-10-135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37dd/2939358/44017435267c/in-10-135-g005.jpg

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