Eye Clinic, Clinic Centre Niš, Bulevar dr Zorana Đinđića 48, 18000 Niš, Serbia.
Bosn J Basic Med Sci. 2010 Aug;10(3):265-9. doi: 10.17305/bjbms.2010.2698.
The aim of our study was to compare levels of antioxidative agent -total SH groups and the final product of lipid peroxidation- malondialdehyde (MDA) in serum, and glutathione (GSH) and MDA in nucleocortical parts of lens after extracapsular extraction of cataract. Patient were (38 with cataract and 38 controls) matched by sex and years of life. Diagnosis of cataract was established by complete ocular examination. All results are expressed as mean ± S.D. A Student's t-test was used to estimate differences between the groups. The level of significance was p<0.05. Total sulfhydryl groups were determined in serum by the method of Ellman as well as GSH content in nucleocortical parts of lenses using the method of Sedlak and Lindsay. Lipid peroxidation, evidenced by formation of thiobarbituric acid reactive substances (TBARS), was determined in nucleocortical parts of the lens and in serum. Our results show a statistical significance in concentration of total SH groups (225.37±82.19µmol/L, controls 311.03±60.37µmol/L p<0.05) and MDA (20.24±8.12, and controls 8.73±2.53µmol/L, p<0,001) in serum among patients with age related cataract and controls. There was no statistical significance in concentration of total SH groups and MDA in serum among patients with different type of age related cataract and in nucleocortical parts of lens. The present study concludes that there is a statistical significance in concentration of total SH groups and MDA in serum among patients with age related cataract and controls, but there were no statistical significance in concentration of GSH and MDA in serum and nucleocortical parts of lens in patient with different type of cataract.
本研究旨在比较白内障囊外摘出术后血清中总巯基（total SH groups）和脂质过氧化终产物丙二醛（MDA），以及晶状体核皮质中谷胱甘肽（GSH）和 MDA 的水平。患者按性别和年龄与对照组（38 例白内障患者和 38 例对照者）匹配。白内障的诊断通过全面眼部检查确定。所有结果均表示为平均值±标准差。采用 Student's t 检验比较两组间的差异。显著性水平为 p<0.05。血清中总巯基的测定采用 Ellman 法，晶状体核皮质中 GSH 的含量采用 Sedlak 和 Lindsay 法。采用硫代巴比妥酸反应性物质（TBARS）法测定晶状体核皮质中的脂质过氧化产物。我们的结果显示，年龄相关性白内障患者血清中总巯基（225.37±82.19µmol/L，对照组 311.03±60.37µmol/L，p<0.05）和 MDA（20.24±8.12，对照组 8.73±2.53µmol/L，p<0.001）的浓度存在统计学意义。但在不同类型的年龄相关性白内障患者的血清和晶状体核皮质中，总巯基和 MDA 的浓度无统计学意义。本研究表明，年龄相关性白内障患者和对照组血清中总巯基和 MDA 的浓度存在统计学意义，但不同类型白内障患者血清和晶状体核皮质中 GSH 和 MDA 的浓度无统计学意义。
