Mumtaz Laraib, Farid Arshad, Yousef Alomar Suliman, Ahmad Naushad, Nawaz Asif, Andleeb Saadia, Amin Adnan
Gomal Centre of Biochemistry and Biotechnology(GCBB), Gomal University, KPK, 29050 D.I.Khan, Pakistan.
Gomal Centre of Pharmaceutical Sciences, Faculty of Pharmacy, Gomal University, D.I.Khan 29050, Pakistan.
Saudi J Biol Sci. 2023 Sep;30(9):103743. doi: 10.1016/j.sjbs.2023.103743. Epub 2023 Jul 20.
Several types of microbial infections are caused by that has developed resistance to antimicrobial agents We therefore investigated the role of plant polyphenols against using in silico and models. The clinical strains of were investigated for determination of resistance pattern and resistance mechanisms including efflux pump, extended spectrum beta lactamase, phenotype detection of AmpC production, and Metallo-β-lactamase. The polyphenolic compounds were docked against transcription regulator BfmR (PDB ID 6BR7) and antimicrobial, antibiofilm, and anti-quorum sensing activities were performed. The antibiogram studies showed that all isolated strains were resistant. Strain A77 was positive in Metallo-β-lactamase production. Similarly, none of strains were producers of AmpC, however, A77, A76, A75 had active efflux pumps. Molecular docking studies confirmed a strong binding affinity of Rutin and Catechin towards transcription regulator 6BR7. A significant antimicrobial activity was recorded in case of quercetin and syringic acid (MIC 3.1 µg/mL) followed by vanillic acid and caffeic acid (MIC 12.5 µg/mL). All tested compounds presented a strong antibiofilm activity against strain A77 (65 to 90%). It was concluded that all tested polyphenols samples posess antimicrobial and antibiofilm activities, and hence they may be utilized to treat multidrug resistance infections.
几种类型的微生物感染是由对抗菌剂产生耐药性的[具体微生物名称未给出]引起的。因此，我们使用计算机模拟和体外模型研究了植物多酚对[具体微生物名称未给出]的作用。对[具体微生物名称未给出]的临床菌株进行了耐药模式和耐药机制的测定，包括外排泵、超广谱β-内酰胺酶、AmpC产生的表型检测以及金属β-内酰胺酶。将多酚类化合物与转录调节因子BfmR（PDB ID 6BR7）进行对接，并进行了抗菌、抗生物膜和群体感应抑制活性实验。抗菌谱研究表明，所有分离菌株均具有耐药性。菌株A77产生金属β-内酰胺酶呈阳性。同样，所有菌株均不产生AmpC，但A77、A76、A75具有活跃的外排泵。分子对接研究证实芦丁和儿茶素对转录调节因子6BR7具有很强的结合亲和力。槲皮素和丁香酸（MIC 3.1 µg/mL）的抗菌活性显著，其次是香草酸和咖啡酸（MIC 12.5 µg/mL）。所有测试化合物对菌株A77均表现出很强的抗生物膜活性（65%至90%）。得出的结论是，所有测试的多酚样品均具有抗菌和抗生物膜活性，因此它们可用于治疗多重耐药性[具体微生物名称未给出]感染。
