具有纳米颗粒基质敏化的近红外发光氧纳米传感器。

Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.

Anal Chem. 2010 Oct 15;82(20):8446-55. doi: 10.1021/ac1015358.

The development of sensors for noninvasive determination of oxygen levels in live cells and tissues is critical for the understanding of cellular functions, as well as for monitoring the status of disease, such as cancer, and for predicting the efficacy of therapy. We describe such nontoxic, targeted, and ratiometric 30 nm oxygen nanosensors made of polyacrylamide hydrogel, near-infrared (NIR) luminescent dyes, and surface-conjugated tumor-specific peptides. They enabled noninvasive real-time monitoring of oxygen levels in live cancer cells under normal and hypoxic conditions. The required sensitivity, brightness, selectivity, and stability were achieved by tailoring the interaction between the nanomatrix and indicator dyes. The developed nanosensors may become useful for in vivo oxygen measurements.

用于非侵入式测定活细胞和组织中氧水平的传感器的发展对于理解细胞功能至关重要，并且对于监测疾病（如癌症）的状态以及预测治疗效果也很重要。我们描述了这种无毒、靶向和比率的 30nm 氧纳米传感器，它由聚丙烯酰胺水凝胶、近红外（NIR）发光染料和表面共轭的肿瘤特异性肽组成。它们能够在正常和缺氧条件下非侵入式实时监测活癌细胞中的氧水平。通过调整纳米基质与指示剂染料之间的相互作用，实现了所需的灵敏度、亮度、选择性和稳定性。所开发的纳米传感器可能对体内氧测量有用。