Laboratory of Cardiovascular Science, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.
NMR Biomed. 2010 Dec;23(10):1166-72. doi: 10.1002/nbm.1545. Epub 2010 Sep 20.
Labeling of cells with superparamagnetic iron oxide nanoparticles permits cell tracking by (1)H MRI while (31)P MRS allows non-invasive evaluation of cellular bioenergetics. We evaluated the compatibility of these two techniques by obtaining (31)P NMR spectra of iron-labeled and unlabeled immobilized C2C12 myoblast cells in vitro. Broadened but usable (31)P spectra were obtained and peak area ratios of resonances corresponding to intracellular metabolites showed no significant differences between labeled and unlabeled cell populations. We conclude that (31)P NMR spectra can be obtained from cells labeled with sufficient iron to permit visualization by (1)H imaging protocols and that these spectra have sufficient quality to be used to assess metabolic status. This result introduces the possibility of using localized (31)P MRS to evaluate the viability of iron-labeled therapeutic cells as well as surrounding host tissue in vivo.
用超顺磁性氧化铁纳米粒子对细胞进行标记，允许通过（1）H MRI 对细胞进行跟踪，而（31）P MRS 则允许对细胞生物能量学进行非侵入性评估。我们通过获得体外固定化 C2C12 成肌细胞中铁标记和未标记的（31）P NMR 光谱来评估这两种技术的兼容性。虽然获得了较宽但可用的（31）P 光谱，但对应于细胞内代谢物的共振峰面积比在标记和未标记的细胞群之间没有显著差异。我们得出结论，（31）P NMR 光谱可以从用足以允许通过（1）H 成像方案进行可视化的铁标记的细胞获得，并且这些光谱具有足够的质量用于评估代谢状态。该结果提出了使用局部（31）P MRS 来评估体内铁标记治疗细胞以及周围宿主组织的可行性。
