Kannan Narayanan, Renitta Robinson Emilin, Guruvayoorappan Chandrasekharan
Department ofBiotechnology Karunya University Karunya Nagar, Coimbatore, Tamil Nadu, India.
J Basic Clin Physiol Pharmacol. 2010;21(2):157-68. doi: 10.1515/jbcpp.2010.21.2.157.
A methanolic extract of Bauhinia tomentosa was studied for its immunomodulatory, antioxidant, and anti-inflammatory activity in BALB/c mice. Intraperitoneal administration of the extract significantly increased the total leukocyte account (10075 +/- 542 cells/cm2) on the 18th day, bone marrow cellularity (93.1 x 10(5) +/- 3.35 cells/femur), and alpha-esterase positive cells (1332 +/- 32 cells/4000 bone marrow cells) when compared with untreated control mice. The weights of lymphoid organs, such as spleen and thymus, also increased. An evaluation of the antioxidant activity of B. tomentosa revealed that the extract inhibited non-enzymic lipid peroxidation in mouse liver microsomes at all concentrations, with an IC50 value of 90 microg/ml. For nitric oxide radical scavenging activity, the IC50 of B. tomentosa was 65.0 microg/ml, indicating that B. tomentosa is a good antioxidant. The anti-inflammatory effect B. tomentosa also significantly reduced acute inflammation of the paw edema induced by carrageenan and formalin.
对绒毛羊蹄甲的甲醇提取物在BALB/c小鼠体内的免疫调节、抗氧化和抗炎活性进行了研究。与未处理的对照小鼠相比，腹腔注射该提取物在第18天时显著增加了总白细胞计数（10075±542个细胞/cm²）、骨髓细胞密度（93.1×10⁵±3.35个细胞/股骨）以及α-酯酶阳性细胞（1332±32个细胞/4000个骨髓细胞）。脾脏和胸腺等淋巴器官的重量也有所增加。对绒毛羊蹄甲抗氧化活性的评估表明，该提取物在所有浓度下均能抑制小鼠肝微粒体中的非酶脂质过氧化，IC50值为90μg/ml。对于一氧化氮自由基清除活性，绒毛羊蹄甲的IC50为65.0μg/ml，表明绒毛羊蹄甲是一种良好的抗氧化剂。绒毛羊蹄甲的抗炎作用还显著减轻了角叉菜胶和福尔马林诱导的爪部水肿急性炎症。
