Purdue University, Weldon School of Biomedical Engineering, West Lafayette, IN, USA.
Acta Biomater. 2011 Feb;7(2):568-77. doi: 10.1016/j.actbio.2010.09.015. Epub 2010 Sep 18.
The in vitro cytocompatibility of silicate (Laponite clay) cross-linked poly(ethylene oxide) (PEO) nanocomposite films using MC3T3-E1 mouse preosteoblast cells was investigated while cell adhesion, spreading, proliferation and mineralization were assessed as a function of film composition. By combining the advantageous characteristics of PEO polymer (hydrophilic, prevents protein and cell adhesion) with those of a synthetic and layered silicate (charged, degradable and potentially bioactive) some of the physical and chemical properties of the resulting polymer nanocomposites could be controlled. Hydration, dissolution and mechanical properties were examined and related to cell adhesion. Overall, this feasibility study demonstrates the ability of using model Laponite cross-linked PEO nanocomposites to create bioactive scaffolds.
采用 MC3T3-E1 小鼠前成骨细胞研究了硅酸钠（Laponite 粘土）交联聚环氧乙烷（PEO）纳米复合膜的体外细胞相容性，评估了细胞黏附、铺展、增殖和矿化与膜组成的关系。通过结合 PEO 聚合物（亲水性、阻止蛋白质和细胞黏附）和合成层状硅酸盐（带电荷、可降解和潜在生物活性）的优点，可以控制所得聚合物纳米复合材料的一些物理和化学性质。对水合作用、溶解和机械性能进行了研究，并与细胞黏附相关联。总的来说，这项可行性研究证明了使用模型 Laponite 交联 PEO 纳米复合材料制造生物活性支架的能力。
