Research Unit of Modeling, Analysis, and Control Systems, ENIG, Gabes, Tunisia.
J Med Food. 2011 Nov;14(11):1383-8. doi: 10.1089/jmf.2010.0168. Epub 2011 Apr 10.
This study investigated the chemical composition (by using gas chromatography/flame ionization detection and gas chromatography/mass spectrometry, an antioxidant [1,1-diphenyl-2-picrylhydrazyl] [DPPH] radical-scavenging assay, and a 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonate [ABTS] radical cation-scavenging assay) and the antimalarial and cytotoxic activities of essential oil extracted from leaves of Melaleuca armillaris. Thirty-two components representing more than 98% of the total composition of the essential oil were identified. The main components were 1,8-cineole (85.8%), camphene (5.05%), and α-pinene (1.95%). The antioxidant activity by ABTS assay showed a mean (± standard deviation) 50% inhibitory concentration (IC(50)) value of 247.3 ± 3.9 mg/L, and the DPPH assay yielded an IC(50) value of 2183.6 ± 44.3 mg/L. The antimalarial study indicated that the essential oil had mild activity against the chloroquine-resistant Plasmodium falciparum FcB1 strain (IC(50), 27 ± 2 mg/L). The cytotoxic activity of this essential oil was tested against MCF7 human breast cancer cells and was found to be high (IC(50), 12 ± 1 mg/L).
本研究调查了从桃金娘科白千层叶中提取的精油的化学成分（使用气相色谱/火焰离子化检测和气相色谱/质谱、抗氧化[1,1-二苯基-2-苦基肼基] [DPPH]自由基清除测定法和 2,2'-联氮双-3-乙基苯并噻唑啉-6-磺酸[ABTS]自由基阳离子清除测定法）、抗疟和细胞毒性活性。鉴定了 32 种成分，占精油总成分的 98%以上。主要成分是 1,8-桉叶油醇（85.8%）、莰烯（5.05%）和α-蒎烯（1.95%）。ABTS 法测定的抗氧化活性显示 50%抑制浓度（IC50）值的平均值（±标准偏差）为 247.3 ± 3.9 mg/L，DPPH 法的 IC50 值为 2183.6 ± 44.3 mg/L。抗疟研究表明，该精油对氯喹耐药恶性疟原虫 FcB1 株具有温和的活性（IC50，27 ± 2 mg/L）。该精油对 MCF7 人乳腺癌细胞的细胞毒性活性进行了测试，结果发现其活性较高（IC50，12 ± 1 mg/L）。
