豚鼠胃黏膜分散细胞中的细胞环磷酸腺苷

Cellular cyclic AMP in dispersed mucosal cells from guinea pig stomach.

作者信息

Batzri S, Gardner J D

出版信息

Biochim Biophys Acta. 1978 Jun 15;541(2):181-9. doi: 10.1016/0304-4165(78)90391-4.

PMID:208634

Abstract

In dispersed mucosal cells from guinea pig stomach cyclic AMP was increased 4-fold by theophylline, 5-fold by prostaglandin E2, and 10- to 15-fold by histamine. Theophylline augmented the increase in cellular cyclic AMP caused by histamine or prostaglandin E1 and the actions of histamine and prostaglandin E1 were additive. Cellular cyclic AMP was not altered by carbachol, gastrin, secretin, vasoactive intestinal peptide, glucagon, insulin or the octapeptide of cholecystokinin. Metiamide or diphenhydramine but not atropine inhibited the increase in cellular cyclic AMP caused by histamine, but did not alter the concentration of cyclic AMP in control cells or in cells incubated with theophylline or prostaglandin E1.

摘要

在豚鼠胃的分散黏膜细胞中，茶碱使环磷酸腺苷（cAMP）增加4倍，前列腺素E2使其增加5倍，组胺使其增加10至15倍。茶碱增强了组胺或前列腺素E1引起的细胞内cAMP增加，且组胺和前列腺素E1的作用是相加的。卡巴胆碱、胃泌素、促胰液素、血管活性肠肽、胰高血糖素、胰岛素或胆囊收缩素八肽均未改变细胞内cAMP水平。甲硫咪胺或苯海拉明可抑制组胺引起的细胞内cAMP增加，但不改变对照细胞或与茶碱或前列腺素E1孵育的细胞中的cAMP浓度，而阿托品则无此作用。