氮杂环丁烷连接：通过光诱导氮杂环丁烷-烯烃环加成实现快速和选择性蛋白质偶联。
Azirine ligation: fast and selective protein conjugation via photoinduced azirine-alkene cycloaddition.
机构信息
Department of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260, USA.
出版信息
Chem Commun (Camb). 2010 Nov 14;46(42):7993-5. doi: 10.1039/c0cc02863k. Epub 2010 Sep 24.
Abstract
We report a new bioorthogonal ligation reaction between p-nitrodiphenylazirine and dimethyl fumarate. This photoinduced azirine-alkene cycloaddition provides a rapid (~2 min) and highly selective route to protein conjugation at neutral pH and room temperature in biological medium.
摘要
我们报告了一种新的生物正交连接反应，即 p-硝基二苯重氮甲烷与富马酸二甲酯之间的反应。这种光诱导的氮杂环丙烷-烯烃环加成反应在中性 pH 值和室温下的生物介质中提供了一种快速（~2 分钟）且高度选择性的蛋白质偶联途径。
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