一种用于监测免疫介导性关节炎模型中干细胞迁移的光学成像方法。
An optical imaging method to monitor stem cell migration in a model of immune-mediated arthritis.
作者信息
Sutton Elizabeth J, Boddington Sophie E, Nedopil Alexander J, Henning Tobias D, Demos Stavros G, Baehner Rick, Sennino Barbara, Lu Ying, Daldrup-Link Heike E
机构信息
Department of Radiology, University of California, San Francisco, 185 Berry Street, Suite 350, San Francisco, CA 94107-0946, USA.
出版信息
Opt Express. 2009 Dec 21;17(26):24403-13. doi: 10.1364/OE.17.024403.
The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.
这项工作的目的是建立一种光学成像技术，以监测间充质干细胞（MSC）在关节炎关节中的整合情况。我们的方法是首先开发一种用荧光染料DiD标记MSC的技术，然后通过光学图像（OI）中DiD荧光的空间分布来追踪细胞迁移动力学。实验方法首先涉及对用DiD标记的MSC进行体外OI，并进行荧光显微镜测量，以确定信号在细胞内的定位。此后，将DiD标记的MSC注射到多关节炎无胸腺大鼠体内，并在几天内监测实验动物体内的信号定位。实验结果表明，DiD整合到了细胞膜中。通过OI观察到DiD标记的MSC在关节炎踝关节中的定位，表明该方法可用于监测关节炎关节中的MSC。
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