Department of Animal Health, National Veterinary Institute, P.O. Box 750 Sentrum, 0106 Oslo, Norway.
Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146, Dep., N-0033 Oslo, Norway.
Res Vet Sci. 2011 Aug;91(1):32-39. doi: 10.1016/j.rvsc.2010.08.012. Epub 2010 Sep 24.
Paratuberculosis was diagnosed in a goat herd that participated in a sanitation program against Mycobacterium avium subsp. paratuberculosis. The aim of this study was to characterise the development of gamma interferon (IFN-γ) and antibody responses as well as the occurrence of faecal shedding. Faecal culture appeared surprisingly sensitive as about 18% and 40% of the goats were positive at 9 and 15-17 months of age, respectively, and shedding was often seen prior to peripheral immune responses. Peripheral IFN-γ responses were not related to protection as clinical and high shedding goats often had high responses. An IFN-γ response usually preceded a humoral response. However, positive antibody titers could sometimes be seen simultaneously with, and even prior to, IFN-γ responses. In conclusion, faecal culture appeared as sensitive as IFN-γ testing. Furthermore, the antibody ELISA and the IFN-γ assay may perform equally well in an infected herd if surveillance is conducted annually.
分支杆菌病在参与针对鸟分枝杆菌亚种副结核分枝杆菌卫生计划的山羊群中被诊断出来。本研究的目的是描述γ干扰素（IFN-γ）和抗体反应的发展以及粪便排出的情况。粪便培养的敏感性令人惊讶，因为大约 18%和 40%的山羊分别在 9 月龄和 15-17 月龄时呈阳性，并且在外周免疫反应之前经常出现粪便排出。外周 IFN-γ 反应与保护无关，因为临床和高排出的山羊通常具有高反应。IFN-γ 反应通常先于体液反应。然而，有时可以同时看到阳性抗体滴度，甚至先于 IFN-γ 反应。总之，粪便培养与 IFN-γ 检测一样敏感。此外，如果每年进行监测，抗体 ELISA 和 IFN-γ 测定在感染的畜群中可能同样有效。
