Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-5620, USA.
Virology. 2010 Nov 25;407(2):368-73. doi: 10.1016/j.virol.2010.08.032. Epub 2010 Sep 24.
The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, which are classified based on the DNA sequence of their non-coding control regions (NCCRs). The archetype BKV NCCR is divided into five blocks of sequence and rearranged variants contain deletions and duplications of these blocks. In this study, a genetic system was developed and used to identify the major determinant of replication ability in primary renal proximal tubule epithelial cells, the natural host cell of BKV. This system was also used to analyze NCCR variants isolated from an immunocompromised patient which contain assorted rearrangement patterns and functional differences. This study solidifies the NCCR as the major genetic determinant of BKV replication ability in vitro.
人类多瘤病毒 BK 病毒（BKV）是一种重要的机会性病原体，其疾病流行率随着免疫功能低下人群的增加而持续上升。迄今为止，尚未正式证明细胞培养中复制的主要决定因素。BKV 存在原始病毒和重排变体，根据其非编码控制区（NCCR）的 DNA 序列进行分类。原始 BKV NCCR 分为五个序列块，重排变体包含这些块的缺失和重复。在这项研究中，开发并使用了一种遗传系统来鉴定原发性肾近端小管上皮细胞（BKV 的天然宿主细胞）中复制能力的主要决定因素。该系统还用于分析来自免疫功能低下患者的 NCCR 变体，这些变体包含各种重排模式和功能差异。这项研究将 NCCR 确定为 BKV 体外复制能力的主要遗传决定因素。
