Mestrado em Ciência Animal, Universidade Paranaense, Praça Mascarenhas de Moraes, 4282, Centro, Umuarama 87.502-210, Paraná, Brazil.
Vet Parasitol. 2010 Dec 15;174(3-4):328-31. doi: 10.1016/j.vetpar.2010.08.039. Epub 2010 Sep 24.
Synanthropic rodents, mainly rats and mice, become ecologically associated with men due to changes in their ecosystems caused by human activities. These animals may take part in the epidemiological cycles of several diseases, including toxoplasmosis. The presence of serum antibodies to Toxoplasma gondii in 43 rodents captured in the urban area of Umuarama, PR, Brazil, was verified by modified agglutination test (MAT). Brain and heart samples were also collected and bioassayed in mice for the isolation of the parasite. Isolated samples were analyzed by 12 multilocus genotyping. Although all rodents were seronegative, the parasite was isolated in one mouse (Mus musculus) and one rat (Rattus rattus). Genotyping showed that these samples were similar to those previously isolated from cats in the state of Parana, Brazil.
由于人类活动导致其生态系统发生变化，共生啮齿动物（主要是鼠类）与人类在生态上产生联系。这些动物可能参与几种疾病的流行病学循环，包括弓形虫病。本研究通过改良凝集试验（MAT）检测了在巴西普鲁登特总统城（Umuarama）市区捕获的 43 只啮齿动物血清中抗刚地弓形虫抗体的存在情况。还采集了脑组织和心肌组织样本，并在小鼠中进行生物测定以分离寄生虫。对分离样本进行了 12 个多位点基因分型分析。尽管所有啮齿动物的血清学检测均为阴性，但在一只小鼠（Mus musculus）和一只大鼠（Rattus rattus）中分离到了寄生虫。基因分型表明，这些样本与之前在巴西巴拉那州从猫中分离到的样本相似。
