Nanobiology Lab, Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, Palampur 176061, HP, India.
Colloids Surf B Biointerfaces. 2011 Jan 1;82(1):224-32. doi: 10.1016/j.colsurfb.2010.08.046. Epub 2010 Sep 9.
The plant isolated antioxidant quercitrin has been encapsulated on poly-d,l-lactide (PLA) nanoparticles by solvent evaporation method to improve the solubility, permeability and stability of this molecule. The size of quercitrin-PLA nanoparticles is 250±68nm whereas that PLA nanoparticles is 195 ± 55nm. The encapsulation efficiency of nanoencapsulated quercitrin evaluated by HPLC and antioxidant assay is 40%. The in vitro release kinetics of quercitrin under physiological condition reveals initial burst release followed by sustained release. Less fluorescence quenching is observed with equimolar concentration of PLA encapsulated quercitrin than free quercitrin. The presence of quercitrin specific peaks on FTIR of five times washed quercitrin loaded PLA nanoparticles provides an extra evidence for the encapsulation of quercitrin into PLA nanoparticles. These properties of quercitrin nanomedicine provide a new potential for the use of such less useful highly active antioxidant molecule towards the development of better therapeutic for intestinal anti-inflammatory effect and nutraceutical compounds.
采用溶剂挥发法将具有抗氧化作用的植物分离得到的槲皮素包封到聚丙交酯-乙交酯共聚物（PLA）纳米粒子中，以提高该分子的溶解度、通透性和稳定性。槲皮素-PLA 纳米粒子的粒径为 250±68nm，而 PLA 纳米粒子的粒径为 195 ± 55nm。通过 HPLC 和抗氧化测定法评估纳米包封槲皮素的包封效率为 40%。在生理条件下，槲皮素的体外释放动力学显示初始突释后持续释放。与等摩尔浓度的游离槲皮素相比，用 PLA 包封的槲皮素观察到的荧光猝灭较少。经过五次洗涤后，载有槲皮素的 PLA 纳米粒子的傅里叶变换红外光谱上出现了槲皮素的特征峰，这为槲皮素被包封到 PLA 纳米粒子中提供了额外的证据。槲皮素纳米药物的这些特性为将这种高活性但用途不大的抗氧化剂分子用于开发更好的治疗肠道抗炎作用和营养化合物的疗法提供了新的潜力。
