Department of Physiological Sciences, Roberto Alcântara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro 20550-030, RJ, Brazil.
J Endocrinol. 2010 Dec;207(3):319-28. doi: 10.1677/JOE-10-0124. Epub 2010 Sep 24.
Resveratrol (Res) has been associated with protective effects against oxidative stress. This study evaluated the effect of Res over lipid peroxidation, antioxidant defense, hepatic sirtuin 1 (SIRT1), which up-regulates antioxidant enzymes, and copper/zinc superoxide dismutase (Cu/Zn SOD) in adult offspring whose mothers were protein restricted during lactation. Lactating Wistar rats were divided into control (C) group, which were fed a normal diet (23% protein), and low-protein and high-carbohydrate (LPHC) group, which were fed a diet containing 8% protein. After weaning (21 days), C and LPHC offspring were fed a normal diet until they were 180 days old. At the 160th day, animals were separated into four groups as follows: control, control+Res, LPHC, and LPHC+Res. Resveratrol was given for 20 days (30 mg/kg per day by gavage). LPHC animals showed a higher total antioxidant capacity (TAC) without change in lipid peroxidation and SIRT1 expression. The treatment with Res increased TAC only in the control group without effect on lipid peroxidation and SIRT1. LPHC animals treated with Res had lower lipid peroxidation and higher protein and mRNA expression of SIRT1 without any further increase in TAC. No significant difference in liver Cu/Zn SOD expression was observed among the groups. In conclusion, maternal protein restriction during lactation programs the offspring for a higher antioxidant capacity, and these animals seem to respond to Res treatment with a lower lipid peroxidation and higher hepatic SIRT1 expression that we did not observe in the Res-treated controls. It is probable that the protective effect can be attributed to Res activating SIRT1, only in the LPHC-programmed group.
白藜芦醇（Res）与抗氧化应激的保护作用有关。本研究评估了白藜芦醇对哺乳期母亲蛋白质限制的成年后代的脂质过氧化、抗氧化防御、上调抗氧化酶的肝 SIRT1（SIRT1）和铜/锌超氧化物歧化酶（Cu/Zn SOD）的影响。哺乳期 Wistar 大鼠分为对照组（C），喂食正常饮食（23%蛋白质）和低蛋白和高碳水化合物组（LPHC），喂食含 8%蛋白质的饮食。断奶（21 天）后，C 和 LPHC 后代喂食正常饮食直至 180 天。在第 160 天，动物分为以下四组：对照组、对照组+Res、LPHC 和 LPHC+Res。白藜芦醇连续给药 20 天（灌胃 30mg/kg/天）。LPHC 动物的总抗氧化能力（TAC）升高，但脂质过氧化和 SIRT1 表达没有变化。Res 处理仅增加了对照组的 TAC，而对脂质过氧化和 SIRT1 没有影响。用 Res 处理的 LPHC 动物的脂质过氧化降低，SIRT1 的蛋白和 mRNA 表达增加，但 TAC 没有进一步增加。各组肝 Cu/Zn SOD 表达无显著差异。结论：哺乳期母亲蛋白质限制使后代具有更高的抗氧化能力，这些动物对 Res 治疗的反应似乎是脂质过氧化降低和肝 SIRT1 表达增加，而在 Res 治疗的对照组中没有观察到这种情况。可能是 Res 通过激活 SIRT1 发挥保护作用，仅在 LPHC 编程组中。
